Treponema Pallidum DNA detection, Mucocutaneous

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General Information

Lab Name
Treponema Pallidum DNA detection, Mucocutaneous
Lab Code
TPLSWB
Epic Name
Treponema pallidum DNA Detection, Mucocutaneous
Description

Detection of Treponema pallidum DNA.

TEMPORARILY UNAVAILABLE, PLEASE SEE Treponema pallidum DNA detection by NAAT [TPLDNA]

This test detects Treponema pallidum, the etiologic agent of syphilis and endemic treponematoses (e.g., yaws and pinta) in mucocutaneous swabs and amniotic fluid. It can be ordered as a primary diagnostic when syphilis is suspected.

Please note that this assay does NOT distinguish subspecies of T. pallidum.

Synonyms
23S LAMP, 23S PCR, chancre, darkfield microscopy, FTA-ABS, meningovascular, neurosyphilis, RPR, spirochete, Syphilis, tabes dorsalis, TP-PA, TPPA, Treponema, Treponema pallidum, treponeme, VDRL
Components
Code Name
TPMC23 T pallidum NAAT: Detection, 23S rDNA
TPMCID T pallidum NAAT: Identification
TPMCSI T pallidum NAAT: Specimen Description
TPMCSP T pallidum NAAT: External Identifier
TPMCSR T pallidum NAAT: Special Requests
TPMCSC T pallidum NAAT: Specimen Comments
TPMCNA T pallidum NAAT: Specimen DNA Extraction
TPMCRV T pallidum NAAT: Pathologist Review
TPMCME T pallidum NAAT: Method Note

Interpretation

Guidelines
Method

DNA extraction, nucleic acid purification, Loop Mediated Isothermal Amplification

Reference Range
See individual components

Ordering & Collection

Specimen Type
UTM Swab, amniotic fluid
Collection

Preferred specimen: Mucocutaneous swabs (including genital, oral, anal/rectal and lesions) and amniotic fluid.

Acceptable specimen: Mucocutaneous swabs (including genital, oral, anal/rectal and lesions) and amniotic fluid.

Amniotic fluid; please provide separate aliquot of Amniotic fluid (min 0.5mL, requested 0.7mL). For other molecular microbiology orders on amniotic fluid, please provide a separate aliquot (2.0mL recommended, 1mL min). If unable to aliquot or if volume is < 2mL, please contact molmicdx@uw.edu for director consult.

Unacceptable specimen:

  • Citrated or heparinized solutions
  • Tissues floating in formalin
  • Swab/fluid collected in tube containing agar
Handling Instructions

Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.

Quantity
Requested: 650uL
Minimum: 500uL

Processing

Receiving Instructions

UWMC/HMC/NW: Temporarily unavailable, please order TPLDNA. Store and send UTM swabs refrigerated or frozen. If specimen storage and transport will exceed 48 hours, freeze at -20 to -80°C.

Misc Sendout

Performance

Lab Department
Micro Molecular Diag(MMD)
Frequency
Mondays and Thursdays. Batched and currently performed 1 – 2 times per week.
Available STAT?
No
Performing Location(s)
CL Molecular Microbiology
206-598-5735

Shipping Address

Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW
Room 171A
Renton, WA 98057

Performing Lab Address

UW Medicine Central Laboratory
Molecular Microbiology
1601 Lind Ave SW
Room 485
Renton, WA 98057

We receive specimens 7 days a week, 365 days a year.

Please e-mail us with any questions or comments you have, so that we can provide you with the best possible service. Our e-mail is monitored in real time, and your inquiry will be answered as soon as possible.

e-mail: molmicdx@uw.edu

For urgent inquiries, billing inquiries, or requests for faxed reports, please contact our Client Services Department at (206) 598-5735 or (800) 713-5198.

Billing & Coding

CPT Codes
87798
LOINC
41163-7
Interfaced Order Code
UOW5582