Treponema Pallidum DNA detection, Mucocutaneous
General Information
- Lab Name
- Treponema Pallidum DNA detection, Mucocutaneous
- Lab Code
- TPLSWB
- Epic Name
- Treponema pallidum DNA Detection, Mucocutaneous
- Description
Detection of Treponema pallidum DNA.
TEMPORARILY UNAVAILABLE, PLEASE SEE Treponema pallidum DNA detection by NAAT [TPLDNA]
This test detects Treponema pallidum, the etiologic agent of syphilis and endemic treponematoses (e.g., yaws and pinta) in mucocutaneous swabs and amniotic fluid. It can be ordered as a primary diagnostic when syphilis is suspected.
Please note that this assay does NOT distinguish subspecies of T. pallidum.
- Synonyms
- 23S LAMP, 23S PCR, chancre, darkfield microscopy, FTA-ABS, meningovascular, neurosyphilis, RPR, spirochete, Syphilis, tabes dorsalis, TP-PA, TPPA, Treponema, Treponema pallidum, treponeme, VDRL
- Components
-
Code Name TPMC23 T pallidum NAAT: Detection, 23S rDNA TPMCID T pallidum NAAT: Identification TPMCSI T pallidum NAAT: Specimen Description TPMCSP T pallidum NAAT: External Identifier TPMCSR T pallidum NAAT: Special Requests TPMCSC T pallidum NAAT: Specimen Comments TPMCNA T pallidum NAAT: Specimen DNA Extraction TPMCRV T pallidum NAAT: Pathologist Review TPMCME T pallidum NAAT: Method Note
Interpretation
- Guidelines
- Method
DNA extraction, nucleic acid purification, Loop Mediated Isothermal Amplification
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- UTM Swab, amniotic fluid
- Collection
-
Preferred specimen: Mucocutaneous swabs (including genital, oral, anal/rectal and lesions) and amniotic fluid.
Acceptable specimen: Mucocutaneous swabs (including genital, oral, anal/rectal and lesions) and amniotic fluid.
Amniotic fluid; please provide separate aliquot of Amniotic fluid (min 0.5mL, requested 0.7mL). For other molecular microbiology orders on amniotic fluid, please provide a separate aliquot (2.0mL recommended, 1mL min). If unable to aliquot or if volume is < 2mL, please contact molmicdx@uw.edu for director consult.
Unacceptable specimen:
- Citrated or heparinized solutions
- Tissues floating in formalin
- Swab/fluid collected in tube containing agar
- Handling Instructions
Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.
- Quantity
-
Requested: 650uL
Minimum: 500uL
Processing
- Receiving Instructions
UWMC/HMC/NW: Temporarily unavailable, please order TPLDNA. Store and send UTM swabs refrigerated or frozen. If specimen storage and transport will exceed 48 hours, freeze at -20 to -80°C.
- Misc Sendout
Performance
- Lab Department
- Micro Molecular Diag(MMD)
- Frequency
- Mondays and Thursdays. Batched and currently performed 1 – 2 times per week.
- Available STAT?
- No
- Performing Location(s)
-
CL Infectious Disease Division (IDD) - Molecular Microbiology
206-520-4600----------------------------------------
Shipping Address
Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW Room 117
Renton, WA 98057
Phone: 206-598-5735Performing Lab Address
UW Medicine Central Laboratory
IDD-Molecular Microbiology
1601 Lind Ave SW Room 485
Renton WA 98057
Phone: (206) 485-9108Contact Information
Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible.
email: molmicdx@uw.edu
The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT.
Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198.
For results or other inquiries, we can be reached by phone at the following numbers:
- Phone: (206) 598-5735
- FAX: (206) 520-4903
For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190
Billing & Coding
- CPT Codes
- 87798
- LOINC
- 41163-7
- Interfaced Order Code
- UOW5582