Enterobacterales DNA Detection

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General Information

Lab Name
Enterobacterales DNA Detection
Lab Code
ENBDNA
Epic Ordering
Enterobacterales DNA Detection
Description

Enterobacterales DNA Detection by PCR is only available as a reflexive test when results indicate the presence of bacteria in the Order Enterobacterales from Bacterial DNA Detection by PCR (BCTDNA) or Bacterial detection by 16S NGS (NGS16S).

Results in 2-3 business days after reflex.

The family Enterobacteriaceae (within the order Enterobacterales) contains over 60 genera and more than 250 species, and organisms within this family include pathogenic bacteria capable of causing many different infectious processes, such as sepsis, pneumonia, urinary tract infections, intra-abdominal infections and foodborne illnesses. 16S gene sequencing, a gold standard for determining the species of a bacteria, however is not adequate due to strong conservation of sequences among members of this family across multiple genera. Instead, sequencing housekeeping genes such as dnaJ has been shown to distinguish species within this family.

We have designed an Enterobacterales PCR test (ENBDNA) based on partial dnaJ gene sequencing which can be used to detect and identify members of Enterobacterales order. This test is currently offered reflexively when 16S sequence (from BCTDNA or NGS16S) suggests presence of Enterobacteriaceae family DNA.

Synonyms
16S sequencing, bacteria 16S, bacteria identification, Bacteria PCR, bacteria sequencing, bacterial 16S, bacterial identification, Bacterial PCR, bacterial sequencing, broad range bacteria PCR, broad range bacterial PCR, dnaJ, dnaJ identification, dnaJ PCR, dnaJ sequening, Enterobacteriaceae identification, Enterobacteriaceae sequencing, Family Enterobacteriaceae, molecular bacteria, molecular bacterial, Synonyms: 16S PCR, universal bacteria PCR, universal bacterial PCR
Components

Ordering & Collection

Specimen Type
This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).
Collection

This only available as a reflexive test, please refer to Bacterial PCR reflex NGS [BCTDNA].

Handling Instructions

This only available as a reflexive test, please refer to Bacterial PCR reflex NGS [BCTDNA].

Quantity
requested: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).
minimum: This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).

Processing

Processing

This only available as a reflexive test, please refer to Bacterial PCR reflex NGS [BCTDNA].

Performance

LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
UW-MT Microbiology, Molecular Diagnostics
206-520-4600

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Shipping Address

Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW Room 117
Renton, WA 98057
Phone: 206-520-4600
Alternate phone: 206-598-6147

Performing Lab Address

Clinical Microbiology Lab, NW177
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195
Phone: 206-598-5735
Alternate phone: 206-598-6147

Contact Information

Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible.

email: molmicdx@uw.edu

The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT.

Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198.

For results or other inquiries, we can be reached by phone at the following numbers:

  • Phone: (206) 598-5735
  • Alternate phone: (206) 598-6147
  • FAX: (206) 520-4903

For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190
Frequency
Results in 2-3 business days after reflex from Bacterial PCR (BCTDNA) or Bacterial detection by 16S NGS (NGS16S).
Available STAT?
No

Billing & Coding

CPT codes
87801
LOINC
76575-0
Interfaced Order Code
UOW4340