Bacterial detection by 16S Next Generation Sequencing is only available as a reflexive test from Bacterial PCR reflex NGS [BCTDNA] when there is evidence of multiple bacterial templates present.
Results in 10 business days after reflex from Bacterial PCR reflex NGS [BCTDNA]
16S rRNA gene sequencing can be used to perform accurate genus- or species-level taxonomic assignment. The 16S gene is sufficiently conserved among bacteria such that we can classify many bacterial species with high specificity and sensitivity. Unfortunately, PCR amplification from polymicrobial specimens is problematic: the presence of multiple templates results in superimposed Sanger reads that are generally uninterpretable. Next-generation sequencing methods provide individual sequence data for millions of DNA molecules, allowing each to be classified independently. This permits bacterial species identification for samples from which usable data cannot be obtained by conventional clinical methods. Libraries of DNA fragments spanning 16S ribosomal RNA gene part of 16S Ribosomal RNA gene are amplified by PCR from bacterial DNA using primers that include adaptor sequences needed for sequencing. Libraries from individual patient samples can be tagged with a unique molecular barcode, allowing multiple samples to be combined in a single run.
Code | Name |
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NG16SI | 16S Gene Next Gen Seq Specimen |
NG16RS | 16S Gene Next Gen Seq Results |
NG16IN | 16S Gene Next Gen Seq Interpretation |
NG16SR | 16S Gene Next Gen Seq Requests |
NG16ME | 16S Gene Next Gen Seq Method |
DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), next-generation sequencing
This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).
UW-MT |
Microbiology, Molecular Diagnostics
206-520-4600 ---------------------------------------- Shipping Address Attn: Molecular Microbiology Performing Lab Address Clinical Microbiology Lab, NW177 |
Contact Information Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible. email: molmicdx@uw.edu The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT. Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198. For results or other inquiries, we can be reached by phone at the following numbers:
For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190 |
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