Bacterial detection by 16S NGS

General Information

Lab Name
Bacterial detection by 16S NGS
Lab Code
NGS16S
Epic Name
Bacterial Detection By 16S NGS
Description

Bacterial detection by 16S Next Generation Sequencing is only available as a reflexive test from Bacterial PCR reflex NGS [BCTDNA] when there is evidence of multiple bacterial templates present.

Results in 10 business days after reflex from Bacterial PCR reflex NGS [BCTDNA]

16S rRNA gene sequencing can be used to perform accurate genus- or species-level taxonomic assignment. The 16S gene is sufficiently conserved among bacteria such that we can classify many bacterial species with high specificity and sensitivity. Unfortunately, PCR amplification from polymicrobial specimens is problematic: the presence of multiple templates results in superimposed Sanger reads that are generally uninterpretable. Next-generation sequencing methods provide individual sequence data for millions of DNA molecules, allowing each to be classified independently. This permits bacterial species identification for samples from which usable data cannot be obtained by conventional clinical methods. Libraries of DNA fragments spanning 16S ribosomal RNA gene part of 16S Ribosomal RNA gene are amplified by PCR from bacterial DNA using primers that include adaptor sequences needed for sequencing. Libraries from individual patient samples can be tagged with a unique molecular barcode, allowing multiple samples to be combined in a single run.

Synonyms
16s deep sequencing, bacterial identification, polymicrobial sequencing
Components

Interpretation

Guidelines
Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), next-generation sequencing

Reference Range
See individual components

Ordering & Collection

Specimen Type
This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).

Processing

Receiving Instructions

This only available as a reflexive test, please refer to Bacterial DNA Detection by PCR (BCTDNA).

Misc Sendout

Performance

Lab Department
Micro Molecular Diag(MMD)
Frequency
Result in 10 business days after reflex from Bacterial DNA Detection by PCR (BCTDNA).
Available STAT?
No
Performing Location(s)
UW-MT Microbiology, Molecular Diagnostics
206-520-4600

----------------------------------------

Shipping Address

Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW Room 117
Renton, WA 98057
Phone: 206-520-4600
Alternate phone: 206-598-6147

Performing Lab Address

Clinical Microbiology Lab, NW177
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195
Phone: 206-598-5735
Alternate phone: 206-598-6147

Contact Information

Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible.

email: molmicdx@uw.edu

The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT.

Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198.

For results or other inquiries, we can be reached by phone at the following numbers:

  • Phone: (206) 598-5735
  • Alternate phone: (206) 598-6147
  • FAX: (206) 520-4903

For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190

Billing & Coding

CPT Codes
87801
LOINC
66885-5
Interfaced Order Code
UOW2766