Anaplasma phagocytophilum Antibody, IgG (Sendout)

General Information

Lab Name

Lab Code

216

External Test Id

ANAP

Description

Useful For:

As an adjunct in the diagnosis of human granulocytic ehrlichiosis
Seroepidemiological surveys of the prevalence of the infection in certain populations

Testing Algorithm:

Mayo Acute Tick-Borne Disease Testing Algorithm

Synonyms

ANAP, Anaplasma phagocytophilum, Ehrlichiosis, HGA, HGE, Human Granulocytic Anaplasmosis, Human Granulocytic Ehrlichiosis

Interpretation

Method

Immunofluorescence Assay (IFA)

Ref. Range Notes

Reference Values: <1:64

Interpretation:

A positive result of an immunofluorescence assay (IFA) test (titer > or =1:64) suggests current or previous infection with human granulocytic ehrlichiosis. In general, the higher the titer, the more likely it is that the patient has an active infection.

Seroconversion may also be demonstrated by a significant increase in IFA titers.

Interferences and Limitations

Cautions:

Previous episodes of human granulocytic ehrlichiosis may produce a positive serologic result.

In rare instances, clinical evidence of infection may also be derived by direct microscopic examination of Giemsa- or Diff-Quik-stained peripheral blood buffy coat smears, which may reveal clusters of round, dark-purple stained, small dots, or clusters of dots (morulae) in the cytoplasm of polymorphonuclear cells. However, this is a very insensitive method.

Performance characteristics have not been established for hemolyzed or lipemic specimens.

Guidelines

Washington DOH Notifiable Conditions

Ordering & Collection

Specimen Type: Blood
Collection: Collect 3 mL blood in RED TOP tube or GOLD SST
Handling Instructions: Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, or heat-inactivated specimen.
Quantity: requested: 0.5 mL serum
minimum: 0.4 mL serum

Processing

Processing

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Login: SPMSND-;REFRIG

MSNDTO: MAYOSD
MSPTYP: SERUM
MTSTRQ: ;Anaplasma phagocytophilum IgG (Mayo Test ANAP)

Sendouts:

Order Mayo Test: ANAP.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, or heat-inactivated specimen.

Performance

LIS Dept Code

Performing Location(s)

Sendout	Mayo Clinic Laboratories (Superior Drive) 800-533-1710 3050 Superior Drive NW Rochester, MN 55901