Anaplasma phagocytophilum Antibody, IgG (Sendout)

Useful For:

• As an adjunct in the diagnosis of human granulocytic ehrlichiosis
• Seroepidemiological surveys of the prevalence of the infection in certain populations

Testing Algorithm:

• Mayo Acute Tick-Borne Disease Testing Algorithm

Reference Values: <1:64

Interpretation:

A positive result of an immunofluorescence assay (IFA) test (titer > or =1:64) suggests current or previous infection with human granulocytic ehrlichiosis. In general, the higher the titer, the more likely it is that the patient has an active infection.

Seroconversion may also be demonstrated by a significant increase in IFA titers.

Cautions:

Previous episodes of human granulocytic ehrlichiosis may produce a positive serologic result.

In rare instances, clinical evidence of infection may also be derived by direct microscopic examination of Giemsa- or Diff-Quik-stained peripheral blood buffy coat smears, which may reveal clusters of round, dark-purple stained, small dots, or clusters of dots (morulae) in the cytoplasm of polymorphonuclear cells. However, this is a very insensitive method.

Performance characteristics have not been established for hemolyzed or lipemic specimens.

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, or heat-inactivated specimen.

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Login: SPMSND-;REFRIG

• MSNDTO: MAYOSD
• MSPTYP: SERUM
• MTSTRQ: ;Anaplasma phagocytophilum IgG (Mayo Test ANAP)

Sendouts:

• Order Mayo Test: ANAP.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, or heat-inactivated specimen.