DAT Negative Hemolytic Anemia Workup (Sendout)

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General Information

Lab Name
Lab Code
1110
Description

Test Description and Utilization:

  • Useful for diagnosing immune hemolytic anemia not detectable by routine methods.
  • Testing will be performed by American Red Cross Pomona Immunohematology Reference Lab.
  • This workup includes Monospecific anti-IgG and anti-C3 by standard methods, DAT anti-IgA, DAT anti-IgM, 4C DAT anti-IgG (low affinity IgG), and Polybrene DAT anti-IgG (low concentration IgG).

Ordering Requirements:

  • Laboratory Medicine Resident (LMR) approval is required. Contact the LMR via the paging operator at (206) 598-6190.
  • Patients should have a concurrent order for a DAT or have a documented negative DAT in the UW Medicine system within the last week.
    • If the recent DAT is negative, then the LMR will approve sendout testing.
    • If the recent DAT is positive but the provider would still like to send this testing for additional information, the clinical utility can be determined by the resident.
  • Providers must submit a completed American Red Cross Immunohematology Consultation Request Form with all orders.
Forms & Requisitions
Synonyms
AIHA, DAT-negative Autoimmune Hemolytic Anemia, Low-affinity antibody, Super Coombs, Supercoombs
Components

Interpretation

Method

Standard tube technique with anti-IgG, -IgM, and -IgA reagents, Polybrene (resuspension and anti-IgG), and/or cold LISS wash technique as needed.

Reference Range
Ref. Range Notes

See report.

Interferences and Limitations

Cautions: The presence of recently transfused RBCs may compromise results for the purpose of diagnosing autoimmune hemolytic anemia.

Ordering & Collection

Specimen Type
Blood
Collection

Collect 6 mL blood in LAVENDER TOP (EDTA) tube

Preferred: Samples collected prior to transfusion.

Forms & Requisitions
Approval Required
Laboratory Medicine Resident (LMR) approval is required.
Handling Instructions

Outside Laboratories: This test is offered to UW Medicine patients only. Outside laboratories should arrange for testing directly with American Red Cross.

Quantity
Requested: 6 mL EDTA whole blood
Minimum: 5 mL EDTA whole blood

Processing

Receiving Instructions

Store and transport whole blood at ambient temperature if shipping same-day (preferred).

  • If sample is drawn concurrently with in-house DAT, the specimen should refrigerated until it can be sent to ARC, pending the in-house DAT results and LMR approval.

Log: SEND1-;AMBIENT

  • RSNDT1: REDCRS
  • RSTYP1: WB
  • RTSRQ1: ;DAT Negative Hemolytic Anemia Workup

Sendouts:

  • Providers must submit a completed ARC Immunohematology Consultation Request Form.
    • Affix CAST Billing/Results labels to the form in the "Submitting Facility" section.
  • Contact the American Red Cross Southern California Lab at 909.859.7410 prior to sending a sample. Record the name of the person contacted and date/time of notification on the ARC IRL request form.
  • Ensure that sample labels include patient name, patient ID number, and date collected.
  • If shipping the same day of collection, send samples at ambient temperature via FedEx Priority Overnight. If shipping the day after collection, send refrigerated.
  • If shipping on a Friday, select "Saturday Delivery" when creating the FedEx label.
  • Shipping address:
    • American Red Cross Blood Services, Southern California Region
    • ATTN: Reference Laboratory
    • 100 Red Cross Circle – Dock C
    • Pomona, CA 91768

Stability: Ambient: Approximately 24 hours; Refrigerated: 7 days; Frozen: Unacceptable.

Misc Sendout

Performance

Lab Department
Frequency
Performed: Monday through Friday. Turnaround Time: 3 Business days from sample receipt at American Red Cross IRL.
Available STAT?
No
Performing Location(s)
Sendout American Red Cross Immunohematology Reference Laboratory
909-859-7410

100 Red Cross Circle
Pomona, CA 91768

Fax: 909-859-7718
Email: PomonaIRL@redcross.org

Billing & Coding

Billing Comments

Price and CPTs vary depending on testing performed as part of the workup.