DAT Negative Hemolytic Anemia Workup (Sendout)

Test Description and Utilization:

• Useful for diagnosing immune hemolytic anemia not detectable by routine methods.
• Testing will be performed by American Red Cross Pomona Immunohematology Reference Lab.
• This workup includes Monospecific anti-IgG and anti-C3 by standard methods, DAT anti-IgA, DAT anti-IgM, 4C DAT anti-IgG (low affinity IgG), and Polybrene DAT anti-IgG (low concentration IgG).

Ordering Requirements:

• Laboratory Medicine Resident (LMR) approval is required. Contact the LMR via the paging operator at (206) 598-6190.
• Patients should have a concurrent order for a DAT or have a documented negative DAT in the UW Medicine system within the last week.
  • If the recent DAT is negative, then the LMR will approve sendout testing.
  • If the recent DAT is positive but the provider would still like to send this testing for additional information, the clinical utility can be determined by the resident.
• Providers must submit a completed American Red Cross Immunohematology Consultation Request Form with all orders.

Collect 6 mL blood in LAVENDER TOP (EDTA) tube

Preferred: Samples collected prior to transfusion.

Store and transport whole blood at ambient temperature if shipping same-day (preferred).

• If sample is drawn concurrently with in-house DAT, the specimen should refrigerated until it can be sent to ARC, pending the in-house DAT results and LMR approval.

Log: SEND1-;AMBIENT

• RSNDT1: REDCRS
• RSTYP1: WB
• RTSRQ1: ;DAT Negative Hemolytic Anemia Workup

Sendouts:

• Providers must submit a completed ARC Immunohematology Consultation Request Form.
  • Affix CAST Billing/Results labels to the form in the "Submitting Facility" section.
• Contact the American Red Cross Southern California Lab at 909.859.7410 prior to sending a sample. Record the name of the person contacted and date/time of notification on the ARC IRL request form.
• Ensure that sample labels include patient name, patient ID number, and date collected.
• If shipping the same day of collection, send samples at ambient temperature via FedEx Priority Overnight. If shipping the day after collection, send refrigerated.
• If shipping on a Friday, select "Saturday Delivery" when creating the FedEx label.
• Shipping address:
  • American Red Cross Blood Services, Southern California Region
  • ATTN: Reference Laboratory
  • 100 Red Cross Circle – Dock C
  • Pomona, CA 91768

Stability: Ambient: Approximately 24 hours; Refrigerated: 7 days; Frozen: Unacceptable.