Beta 2 Microglobulin, Urine (Sendout)

Interpretation

Method

Automated Chemiluminescent Immunometric Assay

Reference Range

See individual components

Ref. Range Notes

Reference Values:

< or =300 mcg/L

Interpretation:

Increased excretion is consistent with renal tubular damage.

Beta-2 microglobulin excretion is increased 100 to 1000 times the upper limit of the reference interval in cadmium-exposed workers.

Interferences and Limitations

Cautions:

Degradation of beta-2 microglobulin occurs at pH less than 6. At the time of urine collection, 1M sodium hydroxide needs to be added as preservative to achieve a pH between 6 and 8.

For diagnostic purposes, the results obtained from this assay should always be used in combination with the clinical examination, patient medical history, and other findings.

References

• Mayo Clinical & Interpretive Information: Beta-2 Microglobulin, Random, Urine

Ordering & Collection

Specimen Type

Urine

Collection

Collection Instructions:

1. Patient should empty bladder.
2. Have patient drink at least 0.5 liters of water.
3. Within 1 hour, collect a random urine specimen.
4. Forward to the laboratory immediately.

Handling Instructions

Outside Laboratories:

• Add 1M NaOH as preservative to random urine collection within 2 hours of collection. This preservative is intended to achieve a pH of between approximately 6 and 8.
• Freeze urine specimen at -20°C while awaiting shipment. Transport on dry ice.

Stability: Frozen (preferred): 14 days; Refrigerated: 48 hours; Ambient: Unacceptable.

Reject Due To: Specimen with pH <6.

Quantity

Requested: 1 mL urine
Minimum: 0.5 mL urine

Processing

Receiving Instructions

SPS: Decant in Sunquest to create an aliquot CID label, transfer urine to a screw-cap plastic vial, and forward the aliquot to the Chemistry manual bench as soon as possible. The pH of the specimen must be adjusted within 2 hours of collection.

Chemistry:

• Add 1 M NaOH to achieve specimen pH of between 6 and 8.
• Freeze urine at -20°C (place in Sendouts Mayo Frozen Rack).

Sendouts:

• Order Mayo Test: B2MU

Stability: Frozen (preferred): 14 days; Refrigerated: 48 hrs; Ambient: Unacceptable.

Reject Due To: Specimen with pH <6.

Misc Sendout

Performance

Lab Department

Sendouts Mayo Lab (FZ)(MAFZ)

Frequency

Performed: Monday, Wednesday, Friday. Reported: 1-3 days from sample receipt at Mayo Clinic Laboratories.

Available STAT?

No

Performing Location(s)

Sendout	Mayo Clinic Laboratories (Superior Drive) 800-533-1710 3050 Superior Drive NW Rochester, MN 55901

Billing & Coding

CPT Codes

82232

LOINC

1953-9

Interfaced Order Code

UOW3997