Soluble IL 2 Receptor (Sendout)

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General Information

Lab Name
Soluble IL 2 Receptor
Lab Code
RSIL2R
Epic Name
Soluble IL-2 Receptor (Sendout)

Also included in the Hyperinflammatory Lab Panel.
External Test Id
2904000
Description

Test Utility: This test is ordered as part of the workup for hyperinflammatory syndromes such as hemophagocytic lymphohistiocytosis (HLH). This test should be ordered concurrently or after Hyperinflammatory Screening Labs have been obtained (CBC with diff, ferritin, fibrinogen, triglycerides, LDH, and D-dimer).

    The following are consistent with, but not specific for, a diagnosis of HLH:

      -Elevated sIL-2R, CXCL-9, and IL-18
      -Elevated sCD163
      -Reduced NK function (or cell surface expression of CD107alpha)
      -Reduced perforin, SAP, or XIAP

        Test Information: The soluble interleukin-2 receptor level is measured by a solid-phase, two-site chemiluminescent immunometric assay. A luminometer reads the light produced, converts it to counts per second, and, using the standard curve recorded in the machine, converts to units per mL (U/mL). Basiliximab binds with the IL-2R in vivo. Results for patients on basiliximab reflect the unbound portion of sIL2R in the plasma sample.

          Ordering Requirements: Submit orders via Epic. A completed Cincinnati Children's Hospital requisition is not required.

          Forms & Requisitions
          Synonyms
          2904000, CD25 Soluble, Hemophagocytic lymphohisticytosis, HLH, Hyperinflammatory Disorder, IL-2R, SIL2R, Soluble CD 25, Soluble CD25, Soluble IL-2R, Soluble Interleukin 2 Receptor
          Components
          Code Name
          RSIL2L Soluble IL 2 Receptor Level
          RSIL2C Soluble IL 2 Receptor Comment

          Interpretation

          Method

          Chemiluminescence

          Reference Range
          See individual components
          Ref. Range Notes

          Reference Interval:

          Age Reference Range
          <3 years: 398 - 1940 U/mL
          3-7 years: 144 - 1329 U/mL
          ≥8 Years: 137 – 838 U/mL

          Ordering & Collection

          Specimen Type
          Blood
          Collection

          Collect 3 mL Whole Blood in EDTA LAVENDER TOP tube

          Forms & Requisitions
          Handling Instructions

          Outside Laboratories: Centrifuge sample and transfer plasma to a separate plastic aliquot. Freeze plasma at immediately at -20°C while awaiting shipment. Transport on dry ice.

          • Note: Plasma must be separated from cells within 24 hours of collection.

          Stability: Frozen (required): 6 months; Ambient: Unacceptable; Refrigerated: Unacceptable.

          Quantity
          Requested: 1 mL EDTA plasma
          Minimum: 0.5 mL EDTA plasma

          Processing

          Receiving Instructions

          Centrifuge sample and transfer plasma to a separate vial; freeze immediately at -20°C.

          • Note: Plasma must be separated from cells within 24 hours of collection.

          Sendouts:

          • Order Cincinnati Children's Test: 2904000.
          • Complete a Cincinnati Children's Diagnostic Immunology Laboratory Requisition.
          • Ship FedEx Priority Overnight, Monday through Thursday only, with sufficient dry ice to ensure that samples remain frozen throughout transport.
            • Package samples as close to FedEx pickup as possible to ensure that samples remain frozen upon arrival at Cincinnati Lab.
            • Samples that arrive thawed will be rejected.
            • Saturday delivery is not available for this laboratory.

          Stability: Frozen (required): 6 months; Refrigerated: Unacceptable; Ambient: Unacceptable.

          Reject Due To: Whole blood sent frozen or 24 hours past stability, blood collected in wrong container/tube type, separated plasma/serum sent room temperature or refrigerated.

          Misc Sendout

          Performance

          Lab Department
          Sendouts(SO)
          Frequency
          Performed: Monday - Friday; Turnaround Time: 30 hours.
          Available STAT?
          No
          Performing Location(s)
          Sendout Cincinnati Children's Laboratories
          513-636-7355

          3333 Burnet Avenue
          Cincinnati, OH 45229

          Billing & Coding

          CPT Codes
          83520
          LOINC
          9654-5
          Interfaced Order Code
          UOW3994