Q Fever IgG and IgM Antibodies (Sendout)

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General Information

Lab Name
Q Fever IgG and IgM Antibodies (Sendout)
Lab Code
RQFP
Epic Ordering
Q Fever IgG and IgM Antibodies (Sendout)
External Test Id
QFP
Description

Useful For: Diagnosis of Coxiella burnetii, the causative agent of Q fever.

Ordering Note: For CAST use only. Ordered reflexively when Q Fever Antibody Screen, Reflex to Titer (Sendout) [RQFVRS] is reactive.

Synonyms
C Burnetii, Coxiella Burnetii Ab, Coxiella Titer, Febrile Agglutinins, OX-19 (Proteus OX-19 - Weil-Felix), OX-2 (Proteus OX-2 - Weil-Felix), OX-K (Proteus OX-K - Weil-Felix), QFever, QFP, Weil-Felix
Components

Interpretation

Method

Indirect Immunofluorescence

Reference Range
See individual components
Ref. Range Notes

Reference Values:

Q Fever Phase I Antibody, IgG: <1:16
Q Fever Phase II Antibody, IgG: <1:16
Q Fever Phase I Antibody, IgM: <1:16
Q Fever Phase II Antibody, IgM: <1:16

Interpretation:

Phase I antibody titers greater than or equal to phase II antibody titers are consistent with chronic infection or convalescent phase Q fever.

Phase II antibody titers greater than or equal to phase I antibody titers are consistent with acute/active infection.

A negative result argues against Coxiella burnetii infection. If early acute Q fever infection is suspected, collect a second specimen 2 to 3 weeks later and retest.

In Q fever sera, it is common to see IgG titers of 1:128 or greater to both phase I and phase II antibody titers. IgG class antibody titers appear very early in the disease, reaching maximum phase II titers by week 8 and persisting at elevated titers for longer than a year. Phase I titers follow the same pattern, although at much lower levels, and may not be initially detected until convalescence.

In Q fever sera, it is common to see IgM titers of 1:64 or greater.

IgM class antibody titers appear very early in the disease, reaching maximum phase II titers by week 3 and declining to very low levels by week 14. Phase I titers follow the same pattern, although at much lower levels, and may not be initially detected until convalescence.

Interferences and Limitations

Cautions: Serologic responses are time dependent. Specimens collected too early in the disease may not have detectable antibody levels. A second specimen collected 2 to 3 weeks may be necessary to detect antibody.

Low level positive titers (ie, <1:256) may remain for prolonged periods of time following resolution of disease.

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in a RED TOP tube or GOLD SST

Handling Instructions
Quantity
requested: 0.5 mL serum
minimum: 0.3 mL serum

Processing

Processing

Orderable as reflex from Q Fever Antibody Screen, Reflex to Titer (Sendout) [RQFVRS] only.

Stability: Refrigerated: 7 days; Frozen: 7 days: Ambient: Unacceptable.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Performance

LIS Dept Code
Sendouts Mayo Lab (RF) (MARF)
Performing Location(s)
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901

Frequency
Performed: Monday - Saturday. Report Available: 1-3 days.
Available STAT?
No

Billing & Coding

CPT codes
86638x4
LOINC
22211-7
Interfaced Order Code
UOW1935