Prospera (Sendout)
General Information
- Lab Name
- Prospera Inhouse Collection
- Lab Code
- RPROSP
- Epic Ordering
- Prospera (Sendout) Panel (O367248)
- External Test Id
- Prospera™
- Description
Test Information: Prospera™ uses single-nucleotide polymorphism (SNP)-based technology to assess for active rejection (AR) by measuring the DNA derived from transplanted donor organs (kidney, heart, lungs).
Ordering Requirements: A Natera Prospera™ kit is required for testing. Providers should arrange with Natera to obtain kits and have the patient bring the kit to Phlebotomy for collection.
- Synonyms
- dd cfDNA, Donor derived cell free DNA, Natera, Prospera Heart, Prospera Kidney, Prospera Lung, RPDDCD
- Components
-
Code Name RPRSPC Prospera Collection
Interpretation
- Method
Single-nucleotide Polymorphism (SNP)
- Reference Range
- See individual components
- Ref. Range Notes
dd-cfDNA Value: Interpretation: ≥1% Increased risk for active rejection <1% Decreased risk for active rejection - Interferences and Limitations
Prospera is contraindicated:
- Less than 24 hours after a biopsy
- Less than 18 hours after dialysis
- In pregnant women
- In recipients of multi-organ or allogeneic cell transplants
- In patients who have received an allograft from a genetically identical twin
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Collect 20 mL blood in 2 x 10 mL Streck Cell-Free DNA BCT® tubes (mottled black and tan) from a Natera Prospera™ kit. Natera will not accept other tubes.
Additional Instructions:
- Fill all tubes completely and mix by gently inverting 8-10 times. Do not shake vigorously.
- Walk samples to the laboratory after collection. Do not transport via pneumatic tube system, as this may compromise the Streck collection tubes.
- When delivering samples to the lab, include the kit and the Natera requisition that prints from Epic.
- Several backup kits may be stored in the lab, but clinics should not rely on DLMP to provide kits due to unknown volume of orders.
- Handling Instructions
Outside Laboratories: Test available to UW Medicine patients only.
- Quantity
-
requested: 20 mL whole blood (2 full Streck tubes) from Natera Prospera™ kit
minimum: 16 mL whole blood (2 Streck tubes with 8mL each) from Natera Prospera™ kit
Processing
- Processing
Store and transport whole blood at room temperature.
- HMC and UW-NW: Forward samples and kit to UW-MT for sendout.
- Fred Hutch: Send samples to Natera using provided kit. Close the order after sendout by entering NATSO for the pending RPROTR (Prospera Sample Tracking) test.
UW-MT Sendouts:
- Package samples along with the Natera requisition (printed from Epic) and ship at room temperature using the provided kit.
- Samples may be shipped on Friday for Saturday delivery.
Stability: Ambient (required): 8 days; Refrigerated: Unacceptable; Frozen: Unacceptable.
CAST:
- Print a pending UNATSO worksheet.
- Use Sunquest 'Audit Trail' to confirm that the sample has been sent out.
- Close the RPROSP order by entering NATSO for the pending RPROTR (Prospera Sample Tracking) component.
- Results will file directly from Natera to Epic via interface.
Performance
- LIS Dept Code
- Sendouts (SO)
- Performing Location(s)
-
Sendout Natera 201 Industrial Road
San Carlos, CA 94070Phone Numbers:
- 650-273-4468 (Organ Health)
- 844-778-4700 (Women's Health)
- 650-489-9050 (Oncology)
- Other Locations/Notes
-
Prospera Clinical Support (Organ Health):
- Phone: 650.273.4468
- Email: transplantclinical@natera.com
- Frequency
- Turnaround Time: 3 days.
- Available STAT?
- No
Billing & Coding
- CPT codes
- Billing Comments
No institutional billing. Patient self-pay or direct insurance billing only.
- LOINC
- 30896-5