HLA Antibody Initial Screen (Sendout)

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General Information

Lab Name
HLA Antibody Initial Screen (Sendout)
Lab Code
RHLASC
Epic Name
HLA Antibody Initial Screen (Sendout)
External Test Id
0000-00HI
Description

Useful For: Initial workup for potential solid organ deceased donor transplant recipients.

    Test Information: Bloodworks Northwest HLA Placeholder code for initial evaluation of potential recipients for deceased donor transplant. Samples submitted to the HLA/Immunogenetics Laboratory will be tested for HLA Antibody Specificity, HLA Class I & II (A,B,C,DR,DQ,DP ) Typing, and ABO group/Rh type. Includes Bloodworks NW tests 3083-16 (HLA Antibody Specificity), 3084-06 (HLA Class I & II Typing), and 3103-00 (ABO Typing) with potential reflex to Anti-A Titer (3115-00A). Anti-A titer (3115-00A) is performed on group B kidney recipients to determine eligibility to receive group A2/A2B kidneys.

      Additional Ordering Guidance: Do not order this test for transplant workups involving a living donor. Refer to alternate placeholders HLA Living Donor Initial Screen, Recipient (Sendout) [RLDSCR] or HLA Living Donor Initial Screen, Donor (Sendout) [RLDSCD].

      References

      Bloodworks Northwest Profile Information:

      Forms & Requisitions
      Synonyms
      0000-00HI, 3083-16, 3084-06, 3103-00, 3115-00A, Deceased Donor Transplant, HLA Antibody Specificity, HLA Class I & II Typing, Initial Transplant Workup
      Components
      Code Name
      RHLCOM HLA Antibody Order Status

      Interpretation

      Method

      This profile may include test(s) performed by the following methods: Molecular; Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens; serial test tube dilution methodology, UNOS protocol using DTT reducing reagent.

      Reference Range
      See individual components
      References

      Bloodworks Northwest Profile Information:

      Ordering & Collection

      Specimen Type
      Blood
      Collection

      5 x 10 mL blood in YELLOW TOP (ACD) tubes and 2 x 10 mL blood in RED TOP (clot) tubes

      • Also Accepted: 5 x 10 mL Yellow ACD and 2 x 6 mL RED (clot) tubes

      Note: Tubes must be filled completely and the collection date and time should be written on the specimen labels.

      Forms & Requisitions
      Handling Instructions

      Outside Laboratories: This test is for use solely with UW Medicine transplant protocols.

      Quantity
      Requested: 50 mL whole blood in YELLOW TOP (ACD) tubes and 20 mL blood in RED TOP (clot) tubes
      Minimum: 40 mL whole blood in YELLOW TOP (ACD) tubes and 10 mL blood in RED TOP (clot) tubes

      Processing

      Receiving Instructions

      Store and transport samples at ambient temperature.

        Sendouts (UW-MT/HMC) and FHCC SPS:

        • Bloodworks NW HLA Test: 0000-00HI
        • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
        • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
        • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
        • Send routine samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
        • STAT orders should be sent to Bloodworks Northwest as received.* Depending on the courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.
          • *STAT orders for this test are rare and require the provider to request approval from the HLA Lab. If approval is obtained, the HLA Lead Technical Coordinator will contact CSS to request expedited transport of a sample. CSS will create an HC1 task for SPS. If this is not done, orders should be considered routine.

        Stability: Ambient

        Misc Sendout

        Performance

        Lab Department
        Sendouts BWNW Lab (AB)(BWAB)
        Frequency
        Performed: Monday through Friday. Reported: Varies depending on test(s) performed.
        Available STAT?
        Yes, only by arrangement with Bloodworks Northwest HLA Lab. Call (206) 689-6580 (or 206-817-7730 after hours) for approval.
        Performing Location(s)
        Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
        206-689-6580

        921 Terry Ave
        6th Floor
        Seattle, WA 98104

        Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
        After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
        Fax: 206-689-6582

        Billing & Coding

        Billing Comments

        Placeholder mnemonic without billing. See individual tests for CPT coding information.