HLA Antibody Check (Sendout)

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General Information

Lab Name
HLA Antibody Check (Sendout)
Lab Code
RHLACK
Epic Name
HLA Antibody Check
External Test Id
0000-00HAU
Description

Useful For: Monitoring of HLA antibodies in potential recipients prior to transplant.

    Test Information: Bloodworks Northwest HLA Placeholder code for periodic monitoring of HLA antibodies in patients awaiting deceased donor transplantation. Samples submitted to the HLA/Immunogenetics Laboratory may be tested for HLA antibody specificity and levels (CPRA) or the serum stored for future testing. Includes Bloodworks NW tests 3083-16 (HLA Antibody Specificity) or 3085-00 (Specimen Storage, Serum), as needed.

      Additional Ordering Guidance: For HLA Antibody Update that includes serum and lymphocyte storage, order alternate placeholder HLA Antibody Check Plus Storage (Sendout) [RHLAST].

      References

      Bloodworks Northwest Test Information: Periodic HLA Antibody Screening of Candidates for Deceased Donors Transplants

      Synonyms
      0000-00HAU, 3083-16, 3085-00, Calculated Percent Reactive Antibody, CPRA, HLA Antibody Specificity, HLA Antibody Update, PRA Update, Pre-Transplant, Solid Organ Transplant
      Components
      Code Name
      RHLCOM HLA Antibody Order Status

      Interpretation

      Method

      Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens

      Reference Range
      See individual components

      Ordering & Collection

      Specimen Type
      Blood
      Collection

      20 mL blood in RED TOP (clot) tubes

      Note: Tubes must be filled completely.

      Forms & Requisitions
      Handling Instructions

      Outside Laboratories: This test is for use solely with UW Medicine transplant protocols.

      Quantity
      Requested: 20 mL whole blood in red top tubes
      Minimum: 10 mL whole blood in red top tube

      Processing

      Receiving Instructions

      Store and transport samples at ambient temperature.

        Sendouts (UW-MT/HMC) and FHCC SPS:

        • Bloodworks NW HLA Test: 0000-00HAU
        • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
        • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
          • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
        • Send routine samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
        • STAT orders should be sent to Bloodworks Northwest as received.* Depending on the courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.
          • *STAT orders for this test are rare and require the provider to request approval from the HLA Lab. If approval is obtained, the HLA Lead Technical Coordinator will contact CSS to request expedited transport of a sample. CSS will create an HC1 task for SPS. If this is not done, orders should be considered routine.

        Stability: Ambient: 10 days.

        Misc Sendout

        Performance

        Lab Department
        Sendouts BWNW Lab (AB)(BWAB)
        Frequency
        Performed: Monday through Friday. Reported: 5-7 business days from sample receipt at Bloodworks NW HLA Lab.
        Available STAT?
        HLA Antibody Specificity may be performed STAT by arrangement with Bloodworks Northwest HLA Lab. Call (206) 689-6580 (or 206-817-7730 after hours) for approval.
        Performing Location(s)
        Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
        206-689-6580

        921 Terry Ave
        6th Floor
        Seattle, WA 98104

        Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
        After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
        Fax: 206-689-6582

        Billing & Coding

        Billing Comments

        Placeholder mnemonic without billing. See individual tests for CPT coding information.