West Nile Virus PCR, CSF (Sendout)
General Information
- Lab Name
- West Nile Virus PCR, CSF
- Lab Code
- RCWNP
- Epic Ordering
- West Nile Virus PCR, CSF (Sendout)
- External Test Id
- WNCSF
- Description
Useful For:
- Rapid testing for West Nile virus (WNV) RNA (lineage 1 and lineage 2) using cerebrospinal fluid specimens
- An adjunctive test to serology for detection of early WNV infection (ie, first few days after symptom onset)
This assay should not be used for screening asymptomatic individuals and should only be used to test patients with signs and symptoms of WNV disease.
- Synonyms
- Arbovirus, Flavivirus, Mosquito Borne Encephalitis, Viral encephalitis, West Nile PCR, WNCSF, WNV
- Components
-
Code Name RCWNVR West Nile Virus PCR Result,CSF
Interpretation
- Method
Real-Time Polymerase Chain Reaction (PCR)
- Reference Range
- See individual components
- Ref. Range Notes
Reference Values: Negative
Interpretation: A positive result indicates the presence of West Nile virus (WNV) RNA and is consistent with early WNV infection.
- Interferences and Limitations
Cautions:
The sensitivity of the assay is very dependent upon the time of illness onset in which the specimen is collected. Polymerase chain reaction testing has the greatest utility when used within the first few days of symptom onset.
A negative test does not exclude infection with West Nile virus (WNV). Therefore, the results obtained should be used in conjunction with clinical findings and serologic test results to make an accurate diagnosis.
This assay detects both viable and nonviable virus. Test performance depends on viral load in the specimen and may not correlate with cell culture performed on the same specimen.
- Guidelines
Ordering & Collection
- Specimen Type
- CSF, Cerebrospinal Fluid
- Collection
-
Collect 0.5 mL CSF in a sterile container
- Handling Instructions
Outside Laboratories: Refrigerate CSF in a sterile vial.
Stability: Refrigerated (preferred): 7 days; Frozen: 7 days; Ambient: Unacceptable.
Reject Due To: Heat-inactivated or centrifuged specimen.
- Quantity
-
requested: 0.5 mL CSF
minimum: 0.3 mL CSF
Processing
- Processing
Refrigerate CSF in a sterile vial.
Sendouts:
- Order Mayo Test: WNCSF.
- Interfaced: Yes.
Stability: Refrigerated (Preferred): 7 days; Frozen: 7 days; Ambient: Unacceptable.
Reject Due To: Heat-inactivated or centrifuged specimen.
Performance
- LIS Dept Code
- Sendouts Mayo Lab (RF) (MARF)
- Performing Location(s)
-
Sendout Mayo Clinic Laboratories
800-533-1710200 First Street Southwest
Rochester, MN 55901 - Frequency
- Performed: Monday - Friday. Report Available: 1-5 days.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 87798
- LOINC
- 34461-4
- Interfaced Order Code
- UOW3533