Delve Detect mNGS, CSF (Sendout)

Interpretation

Guidelines

• Washington DOH Notifiable Conditions

Method

Pathogen agnostic, metagenomic next generation sequencing with bioinformatic analysis and interpretive report.

Reference Range

See individual components

Ref. Range Notes

Reference Range:

DNA Viruses:	Not Detected
RNA Viruses:	Not Detected
Bacteria:	Not Detected
Fungi:	Not Detected
Parasites:	Not Detected

References

• Delve Bio Test Information: DelveDetect, CSF

Ordering & Collection

Specimen Type

CSF

Collection

1 mL CSF collected in a sterile collection container

• CSF to be transferred under sterile conditions by the Microbiology or Molecular Microbiology lab to a dedicated Delve Bio tube with stabilization buffer.

Approval Required

Microbiology/Virology Faculty approval required.

Handling Instructions

Outside Laboratories: Due to special collection requirements, clients should arrange for testing directly with Delve Bio.

Quantity

Requested: 1 mL CSF transferred to a Delve Bio sample tube
Minimum: 0.5 mL CSF transferred to a Delve Bio sample tube

Processing

Receiving Instructions

PSPS: Samples must be aliquoted using aseptic technique. Transport the original CSF container and a new Delve Bio kit to Microbiology laboratory for assistance with sample transfer. If samples are unable to be processed immediately, refrigerate the unopened CSF sample.

Microbiology/Molecular Microbiology Lab:

• A Delve Bio kit is required. Kits are available in PSPS Sendouts.
• Using the materials provided in the kit, transfer 1 mL CSF to the Delve Bio sample tube under sterile conditions (refer to full instructions linked below).
  • Delve Bio Sample Transfer Kit Instructions
• 1mL of CSF is recommended; if less than 1mL is available for transfer, contact Delve at (844) 221-7423 for further instruction.
• Ensure that the Sunquest label does not cover the expiration date on the Delve tube.
• Once samples have been transferred, return the sample and kit to the Sendouts bench. Once transferred to the Delve Tube, store samples at ambient temperature.
• If unable to transfer samples within 6 hours, unopened CSF may be stored refrigerated for up to 7 days (or up to 90 days at -20°C).

Sendouts:

• Delve Bio Test: Delve Detect CSF (DN01)
• CSF must be transferred to a Delve Bio sample tube or the order will be rejected.
• Contact CAST for assistance with online ordering. Delve Detect orders are considered urgent and should be expedited as much as possible.
  • CAST requires the Delve Tube ID (DNT#####) and Manifest ID (DM#####) to place the order. This information is found on the bottom of the barcode label included in the kit.
• Once the order has been placed, CAST will provide an electronic requisition to be be sent with the sample.
• Package sample and TRF in the provided kit. Send only one sample per kit.
  • Delve Bio Kit Sample Transfer Kit Instructions.
• Ship samples Monday through Friday using the provided Delve Bio kit.
• Notify CAST of an outgoing order via Teams, phone, or HC1 Task. Results should be expedited.

Stability (once transferred to the Delve Bio Sample tube): Ambient (preferred): 1 month; Refrigerated: 2 months; Frozen (-20°C): 90 days.

Stability (Prior to Transfer to Delve Bio Sample Tube): Refrigerated: 7 days; Frozen (-20°C): 90 days; Ambient: 6 hours. No more than 2 freeze/thaw cycles.

Reject Due To: Gross hemolysis (>200mg/dl), insufficient volume (<0.5mL), sample not in Delve Tube, leaking sample.

Sample Transfer Kits are provided by Delve Bio: Call (844) 221-7423 or email: support@delve.bio.

Misc Sendout

Performance

Lab Department

Sendouts(SO)

Frequency

Performed: Monday through Saturday. Report Available: 2 business days from sample receipt at Delve Bio.

Available STAT?

No

Performing Location(s)

Sendout	Delve Bio, Broad Clinical Laboratories, LLC 844-221-7423 27 Blue Sky Drive Burlington, MA 01803	Fax: 833-556-5216

Billing & Coding

CPT Codes

81479