BCR/ABL p190, Quant by RT PCR

General Information

Lab Name

Lab Code

QP190

Epic Ordering

BCR/ABL p190 Quantitative

Description

The Philadelphia chromosome (Ph) is the product of the reciprocal translocation between the ABL1 gene on chromosome 9, and the BCR gene on chromosome 22. Depending on variations in splicing, the BCR exon 1 can join to the ABL1 exon 2, resulting in the p190 (e1:a2) BCR/ABL1 breakpoint. p190 is found almost exclusively in Ph+ALL patients, but can, on occasion, be found in CML patients as an alternative splice variant. Our p190 assay is a laboratory-developed test using real- time quantitative PCR.

Reportable Range: 0.01 – 100%
Limit of Detection: 0.002%
Limit of Quantification: 0.01%
Precision near LOD (CV): 24%

Stability is 4 days, specimens received after 4 days will be rejected.

Hematopathology test request form can be found at Request Form.

Synonyms

B-ALL, B-Cell Acute Lymphoblastic Leukemia, BALL, P190, QP190, Quantitative BCR/ABL p190, t(9;22)

Components

Code	Name
P190SD	BCR/ABL p190 Specimen Desc
P190RS	BCR/ABL p190, Quant by RT PCR
P190RA	BCR/ABL p190 Ratio %
P190IN	BCR/ABL p190 Interpretation
P190M	BCR/ABL p190 Method
P190GR	P190 Case Number

Interpretation

Method

LDT, RT-PCR

Reference Range

See individual components

Guidelines

Molecular Testing for Hematopoietic Disorders

Ordering & Collection

Specimen Type: Peripheral blood, Bone Marrow aspirate
Collection: Blood: 6mL in LAVENDER top (EDTA) (minimum total of 5 million WBC)
Bone Marrow: 1-2 mL in a LAVENDER top (EDTA) (minimum total of 5 million WBC)

Unacceptable:
Specimens with low volume or inadequate WBC.
Specimens received beyond stability limit.
Tissue, Decalcified or unacceptable fixatives

Please note: While green top heparin tubes are accepted for testing, there is documentation that heparin can interfere with some PCR.
Forms & Requisitions: Requisition
Handling Instructions: Samples should be transported at RT and arrive in lab within 24 hours.

If sample is expected to arrive in the HP lab beyond 24 hours, store and transport at 2 – 6˚C.
Quantity: requested: Blood: 6mL in EDTA, Bone Marrow: 2mL in EDTA
minimum: Blood: 4mL in EDTA, Bone Marrow: 1mL in EDTA

Processing

Processing: Send specimens to Hematopathology – Molecular department for processing.

The stability limit is 4 days, samples beyond this limit will be rejected.

Performance

LIS Dept Code

Molecular Hematopathology (MHP)

Performing Location(s)

Other	Hematopathology, Molecular 206-606-7060 UW Hematopathology Laboratory, G7-800 Fred Hutchinson Cancer Center 825 Eastlake Avenue E. Seattle, WA 98109	Hematopathology, Molecular hours: 7:00 am - 5:30 pm M-F 8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays

Frequency

Monday, Wednesday, Friday: Results available within 96hrs from receipt.

Available STAT?

Billing & Coding

CPT codes: 81207
LOINC: 21823-0
Interfaced Order Code: UOW2718