NPM1 Quantitative for MRD

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General Information

Lab Name
NPM1 Quantitative for MRD
Lab Code
NPM1NG
Epic Ordering
NPM1 Insertion Mutation by NGS
Description

This quantitative test detects common insertion mutations in NPM1 gene and is intended for post-treatment minimal residual disease monitoring in NPM1-mutated AML. Purified genomic DNA is PCR amplified using double- indexed primers coupled with sequencing adaptors for subsequent next generation sequencing using an Illumina Miseq platform.

Reportable Range: 0.007 – 50.00%
Limit of Detection: 0.003%
Limit of Quantification: 0.007%
Precision near LOD (CV): 54%

Hematopathology test request form can be found at Request Form.

Forms & Requisitions

Requisition

Synonyms
Acute Myeloid Leukemia, AML, NPM, NPM-1, NPM1, NPM1 Exon 12 Insertion mutation, Nucleophosmin
Components

Interpretation

Method

PCR Amplification then Next Generation Sequencing

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Blood, Bone Marrow, Tissue - Fresh, frozen or FFPE/PET (Blocks), Fluids, Slides, Sorted Cells
Collection

Preferred:
Blood: 6mL in Lavender Top (EDTA)

Bone Marrow: 1-2mL in Lavender Top (EDTA)

Tissue, Fluids or Sorted cells: Sterile container in RPMI

Formalin-fixed paraffin embedded tissue (FFPE/PET) block

Slides: 10 unstained

Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.

Extracted DNA from CLIA certified laboratory.

Formalin-fixed paraffin embedded tissue (FFPE/PET)

Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides


Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives

Forms & Requisitions

Requisition

Handling Instructions
  • Deliver samples to Hematopathology as soon as possible.
  • If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
  • Samples stored at room temperature or refrigerated older than 7 days will be rejected.
  • Frozen samples should be shipped on dry ice.
Quantity
requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides

Processing

Processing

Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.

Specimens must be received in lab by 7am on Wednesday to be included on run.

Performance

LIS Dept Code
Molecular Hematopathology (MHP)
Performing Location(s)
Other Hematopathology, Molecular
206-606-7060

UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109

Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays

Frequency
Performed once weekly on Wednesdays; results may take up to 10 days.
Available STAT?
No

Billing & Coding

CPT codes
81310
LOINC
54448-6
Interfaced Order Code
UOW3029