Respiratory MTB Complex PCR

General Information

Lab Name
Respiratory MTB Complex PCR
Lab Code
MTBPC
Epic Name
Respiratory MTB Complex PCR
Description

This test must be ordered with an AFB culture with stain (AFBHSC). Detection of rifampin resistance will be reported if Mycobacterium tuberculosis complex is detected. Exception: outside locations that perform AFB culture may submit sediments of specimens concentrated for AFB culture for testing. This test is reflexively ordered on the first sputum for AFB culture on HMC, UWMC-ML and UWMC-NW patients, except cystic fibrosis patients who have the test performed by request only. Additional tests can be ordered by request as long as the patient has not been on anti-TB medications for longer than 3 days. Reflexive testing is repeated every 28 days on TB negative patients. See also TBCDNA for TB PCR performed at UW-MT Microbiology. For request on FLUIDS or TISSUE see, M. tuberculosis complex DNA Detection [TBCDNA]. Specimen deadlines for same day results are 7 AM @UW-MT and 9 AM @HMC. This test detects M. tuberculosis complex DNA. Specimens are concentrated and cultured (see, AFB Culture with Stain [AFBHSC]).

Synonyms
AFB-Amplified, AMTD, Cepheid, GeneXpert, M. Tuberculosis, MTD, Mycobacteria, PCR-TB, Xpert
Components

Interpretation

Guidelines
Method

Nucleic Acid Amplification (Cepheid GeneXpert).

Reference Range
See individual components

Ordering & Collection

Specimen Type
Sputum, Bronchoalveolar Lavage (BAL), Bronchial Washings
Quantity
Requested: 5-10 mL Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture
Minimum: 2 mL (suboptimal) Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture

Processing

Receiving Instructions

Specimen must be refrigerated within 30 minutes and received within 5 days of collection.

Misc Sendout

Performance

Lab Department
Serology(SR)
Frequency
Monday - Sunday
Available STAT?
No
Performing Location(s)
HMC Microbiology
206-744-5858

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Billing & Coding

CPT Codes
87556
LOINC
85362-2
Interfaced Order Code
UOW5845