Respiratory TB PCR

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General Information

Lab Name
Respiratory TB PCR
Lab Code
MTBP
Epic Ordering
Respiratory TB PCR
Description

This test must be ordered with an AFB culture with stain (AFBHSC). Exception: outside locations that perform AFB culture may submit sediments of specimens concentrated for AFB culture for testing. This test is reflexively ordered on the first sputum for AFB culture on HMC, UWMC-ML and UWMC-NW patients, except cystic fibrosis patients who have the test performed by request only. Additional tests can be ordered by request as long as the patient has not been on anti-TB medications for longer than 3 days. Reflexive testing is repeated every 28 days on TB negative patients. See also TBCDNA for TB PCR performed at UW-MT Microbiology. For request on FLUIDS or TISSUE see TBCDNA. Specimen deadlines for same day results are 7 AM @UW-MT and 9 AM @HMC. This test detects M. tuberculosis complex DNA. Specimens are concentrated and cultured (see AFBHSC).

Synonyms
AFB-Amplified, AMTD, Cepheid, GeneXpert, M. Tuberculosis, MTD, Mycobacteria, PCR-TB, Xpert
Components

Interpretation

Method

Nucleic Acid Amplification(Cepheid GeneXpert)

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Sputum, Bronchoalveolar Lavage (BAL), Brochial Washings
Collection

Deep cough or induced sputum specimens, bronchoalveolar lavage, or bronchial washings

Handling Instructions

Specimen must be refrigerated within 30 minutes and received within 5 days of collection.

Quantity
requested: 5-10 mL Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture
minimum: 2 mL (suboptimal) Sputum, BAL, or Bronchial Washings, or 1 mL sediment from specimen concentrated for AFB culture

Processing

Processing

Specimens are stored refrigerated until concentrated.

Performance

LIS Dept Code
Microbiology (MC)
Performing Location(s)
HMC Microbiology
206-744-5858

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency
Daily
Available STAT?
No

Billing & Coding

CPT codes
87556
LOINC
14556-5
Interfaced Order Code
UOW2952