1:1 Mix

General Information

Lab Name

1:1 Mix

Lab Code

MIX1P

Epic Ordering

1:1 Mix Coag Panel

Description

A mixing study is used to evaluate the cause of prolonged Prothrombin Time (PT), PTT and/or Thrombin Time. A 1:1 mix of the patient's plasma and normal pooled plasma is performed and the PT and/or PTT is measured again. If the PT and/or PTT corrects, this suggests a factor deficiency. If the PT and/or PTT does not correct, this suggests presence of an inhibitor. If Thrombin Time is prolonged due to the presence of heparin in the sample, a heparin neutralization step is performed before running the mixing tests. A mixing study interpretation may direct further coagulation testing.

Synonyms

MIX 1:1 (PRO & PTT & TT & FIBCL)

Components

Code	Name
MIX1	1:1 Mix
PTTPT	PTT Patient
PTTNOR	PTT Normal Pool
PTTMIX	PTT 1:1 Mix Immediate
INCMIX	PTT 1:1 Mix Incubated
PROPT	PT Patient
PRONOR	PT Normal Pool
PROMIX	PT 1:1 Mix
MIXCOM	1:1 Mix Comment
TTPAT	Thrombin Time
FIBCL	Fibrinogen
XMIX	1:1 Mix, 2nd tube

Interpretation

Method: Clot based
Reference Range: See individual components
Ref. Range Notes: For questions regarding interpretation of 1:1 Mix, contact the Lab Med Resident on-call through the paging operator at 206-598-6190.

Ordering & Collection

Specimen Type: Blood
Collection: Two 3 mL plastic BLUE TOP (Citrate) Tubes
Handling Instructions: The Laboratory MUST process specimen, within 6 hours of blood collection.
Quantity: requested: entire sample

Processing

Processing: Take specimen to the Coag bench for processing.

Outside Laboratory: Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 2.0 mL) @ -20°C to -80°C. Send Frozen on dry ice.

Performance

LIS Dept Code

Performing Location(s)

HMC	Coagulation 206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT	Coagulation 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Daily

Available STAT?

Yes, Daily 7 am – 10 pm. For other times, contact the Lab Medicine Resident for approval.

Billing & Coding

CPT codes: 85384, 85610, 85611, 85670, 85730, 85732
LOINC: 18720-3
Interfaced Order Code: UOW5033