Mycobacterium chimaera DNA detection by PCR

General Information

Lab Name

Lab Code

MCHDNA

Epic Name

(not orderable in Epic)

Description

Mycobacterium chimaera DNA detection by PCR is only available upon request as a reflexive test if a 3-way split ID of “Mycobacterium chimaera or Mycobacterium intracellularae or Mycobacterium paraintracellularae” is reported from tissue/fluid or a pure culture.

Results in 2-3 business days after reflex.

Synonyms

Acid fast bacilli PCR, acid fast bacteria, acid-fast bacteria, AFB identification, AFB PCR, AFB sequencing, M. avium complex, MAC, MAI PCR, Mycobacteria PCR, Mycobacterial sequencing, Mycobacterium chimaera, Mycobacterium identification

Components

Code	Name
MCSRRS	M chimaera PCR: Detection
MCHSI	M chimaera PCR: Specimen Description
MCHSPI	M chimaera PCR: External Identifier
MCHSR	M chimaera PCR: Special Requests
MCHSC	M chimaera PCR: Specimen Comments
MCHNAE	M chimaera PCR: Specimen DNA Extraction
MCHREV	M chimaera PCR: Pathologist Review
MCHME	M chimaera PCR: Method Note

Interpretation

Guidelines

Method

DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), sequencing

Reference Range

See individual components

Ordering & Collection

Specimen Type

Tissue (Fresh frozen or paraffin-embedded), Fluid (see Acceptable Specimens for details), Pure Culture

Collection

Acceptable Specimens

For tissue/fluid acceptable specimens please refer to corresponding battery that this test can be reflexed from. Each of the following batteries have a variation in acceptable specimens.

Pure Culture

Plate/slant: should have visible growth
Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis
A mixed culture will be subject to an additional charge

Optimal Quantity

For tissue/fluid Optimal Quantity please refer to corresponding battery that this test can be reflexed from.

Handling Instructions

Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.

Shipping/Handling

Fresh frozen tissue/fluid specimens should be collected into a DNA free container labeled with at least two identifiers and be submitted and maintained on dry ice.
Formalin Fixed Paraffin-embedded tissues (FFPE, PET) can be sent ambient or with ice packs during warmer summer months to prevent melting.
Pure cultures* should be sent at room or refrigerated temperature. Follow approved regulations for transporting infectious substances.

*Plate/slant: should have visible growth
*Broth/positive blood bottles: must have organisms visible by stain to be acceptable for sequence analysis

Quantity

Requested: See Optimal Quantity above
Minimum: Specimens below optimal quantity are acceptable for testing, however, diagnostic yield is generally proportional to specimen size.

Processing

Receiving Instructions: UWMC/HMC/NW: Store and send fresh tissue/fluid specimens refrigerated, if specimen storage and transport will exceed 8 hours, freeze at -20°C. Freeze all fresh tissue/fluid specimens at -20°C upon arrival in UW Molecular Microbiology.

Misc Sendout

Performance

Lab Department

Micro Molecular Diag(MMD)

Frequency

Results in 2-3 business days after reflex.

Available STAT?

Performing Location(s)

CL	Molecular Microbiology 206-598-5735 Shipping Address Attn: Molecular Microbiology UW CLSPS 1601 Lind Ave SW Room 171A Renton, WA 98057 Performing Lab Address UW Medicine Central Laboratory Molecular Microbiology 1601 Lind Ave SW Room 485 Renton, WA 98057	We receive specimens 7 days a week, 365 days a year. Please e-mail us with any questions or comments you have, so that we can provide you with the best possible service. Our e-mail is monitored in real time, and your inquiry will be answered as soon as possible. e-mail: molmicdx@uw.edu For urgent inquiries, billing inquiries, or requests for faxed reports, please contact our Client Services Department at (206) 598-5735 or (800) 713-5198.

Billing & Coding

CPT Codes: 87551
LOINC: 14974-0
Interfaced Order Code: UOW4632