Lupus Anticoagulant Group and Anti phospholipid Panel with interpretation

General Information

Lab Name
Lupus Anticoag/APL Panel with Interpretation
Lab Code
LUPGI3
Epic Name
Lupus Anticoag/APL Panel with Interpretation
Description

Antiphospholipid syndrome (APS) is characterized by one or more clinical manifestations (such as thrombocytopenia, arterial and venous thrombosis and recurrent fetal loss) with persistent laboratory evidence of antiphospholipid antibodies. Antiphospholipid antibodies (APL) are a heterogenous group of antibodies (immunoglobulins [Igs]) directed against phospholipids and phospholipid-binding proteins. APL can be transient (eg, associated with an acute infection) or persistent (present on two or more occasions at least 12 weeks apart). Testing for APL includes Anticardiolipin antibody (IgG or IgM), Anti-beta2 glycoprotein I antibody (IgG or IgM), and Lupus anticoagulant (LA).

The Hexagonal Phase Phospholipid Lupus Anticoagulant assay (HEXLA) is designed for the qualitative detection of Lupus Anticoagulant (LA) in plasma using hexagonal phase phospholipid and LA sensitive APTT reagent. This HEXLA assay is an integrated (screen and confirm) assay for detection of Lupus Anticoagulant. The delta correction of equal to or greater than 6 seconds is determined to be positive for Lupus Inhibitor.

When clinically indicated, Lupus Anticoagulant, DRVVT (LUPAC) will be reflexively added for diagnosis with an additional charge.

NOTE: Results will be reviewed/Interpreted by an MD and a professional fee is billed. If an MD review is not desired, orders may be placed for “Lupus anticoagulant and antiphospholipid panel without interpretation” via LABUND.

Outside clients should fill and submit Coagulation Patient Clinical History Form

Forms & Requisitions

Outside clients should fill and submit Coagulation Patient Clinical History Form

Synonyms
Lupus Anticoagulant and Anti-phospholipid antibodies, Lupus Inhibitor and Anti-phospholipid antibodies, Lupus Inhibitor and Antiphospholipid antibodies
Components

Interpretation

Method

See Individual tests

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood
Collection

Two each of a 3 or 5 mL BLUE TOP Tubes

Forms & Requisitions

Outside clients should fill and submit Coagulation Patient Clinical History Form

Handling Instructions

Laboratory must process the blue top tubes, within 4 hours of blood collection.

If Lupus Anticoagulant and antiphospholipid without interpretation is ordered, order Lab Mnemonic: HEXPLP and APLSP.

Quantity
Requested: Entire Blue top tubes
Minimum: Entire Blue top tubes

Processing

Receiving Instructions

UW-MT: Take Blue tops to UW-MT Coag for processing. Coag tech will freeze TWO plasma aliquots (minimum 1 mL each). One aliquot will go to Immunology and one aliquot should be sent to HMC Coag. If one Blue top is received, make TWO aliquots (minimum 1 mL each). If only 1.0 mL plasma aliquot is available, send aliquot to HMC Coag first with pink shared slip.

HMC: Take both Blue tops to HMC Coag Lab for processing. Coag tech will freeze TWO plasma aliquots (minimum 1 mL each). One aliquot will go to Immunology and one aliquot to HMC Special Coag. If one Blue top is received, make TWO aliquots (minimum 1 mL each). If only 1.0 mL plasma aliquot is available, send aliquot to HMC Special Coag first with pink shared slip.

Outside Laboratory: Centrifuge Blue tops for 10 minutes, remove plasma & respin plasma for another 10 minutes. Decant and freeze TWO plasma aliquots (minimum 1.0 mL each) at -20°C to -80°C. Ship frozen on dry ice.

SPS Note: If two plasma tubes are received, send one to Immunology for APLSP and one to HMC Coag for LUPI. If one serum and one plasma are received, change order to APLSP and LUPI. Use serum tube for APLSP and plasma tube for LUPI. If only one plasma tube is received, order LUPGI2 and send to HMC Coag first with pink shared slip.

Misc Sendout

Performance

Lab Department
Frequency
Lupus Inhibitor: Run Monday, Thursday. Reported by end of day. Anti Phos Group: Monday-Friday
Available STAT?
No
Performing Location(s)
HMC Coagulation, Special
206-520-4600

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

UW-MT Immunology
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Billing & Coding

CPT Codes
85384, 85390, 85598, 85610, 85670, 85730, 86146x2, 86147x2
LOINC
18720-3
Interfaced Order Code
UOW5251