HIV1 RNA Quantitation
General Information
- Lab Name
- HIV1 RNA Quantitation
- Lab Code
- HRTABB
- Epic Ordering
- HIV1 Rna Quantitation
- Description
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® HIV-1 quantitative assay for the detection and quantitation of human immunodeficiency virus type 1 in EDTA plasma using real-time PCR amplification.
The range of quantitation of HIV-1 RNA is 20 to 10,000,000 copies/mL (33 to 1.67 x 107 International Units/mL).
The Roche cobas® HIV-1 quantitative assay uses primers specific for the HIV-1 gag gene and the HIV-1 LTR region (dual target).
- Synonyms
- HIV PCR, HIV Viral Load
- Components
-
Code Name HRTDES HIV RNA Specimen HRRSLT HIV RNA Result HRLOG HIV RNA Copies/mL(Log10) HRINTP HIV RNA Interpretation
Interpretation
- Method
Real-time Polymerase Chain Reaction
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Plasma (EDTA)
- Collection
-
Blood: EDTA plasma or PPT
Unacceptable: plasma from GREEN TOP (Heparin), YELLOW (ACD) or GRAY (Oxylate) and serum (No RED TOP)
- Handling Instructions
Freshly drawn whole blood may be stored and/or transported for up to 24 hours at 2°C to 25°C prior to plasma preparation through centrifugation.
- Quantity
-
requested: 2.0 mL plasma
minimum: 0.75 mL plasma
Processing
- Processing
Blood: Centrifugation upon arrival, remove plasma from cells.
Plasma may be stored and/or transported:
2°C to 8°C for up to 6 days
-18°C ± 2°C for up to 12 weeks
-75°C ± 15°C for long term storage up to 6 months. Avoid multiple freeze/thaw cycles.
Outside Laboratory: Plasma may be stored at 2°C to 8°C for up to 6 days after collection. To ensure testing is completed within this 6-day window, shipment time should not exceed 48 hours from the time of collection, and samples should be shipped on wet ice (ice pack). Alternatively, samples can be frozen at -18°C or colder and shipped on dry ice for extended preservation.
Performance
- LIS Dept Code
- Central Lab Virology (RVIR)
- Performing Location(s)
-
CL Virology, Renton
206-685-66561602 Raymond Ave SW
Renton, WA 98057-3356
206-685-6656 opt 4
- Frequency
- Tuesday - Saturday. Results same day.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 87536
- LOINC
- 49890-7
- Interfaced Order Code
- UOW1094