Heme Amplicon Panel by NGS

General Information

Lab Name

Lab Code

HOTSPT

Epic Name

Heme Amplicon Panel by NGS

Description

UW Heme Amplicon panel is a multiplexed gene panel assay that detects mutations related to treatment, prognosis, or diagnosis in the setting of hematolgical malignancy. The regions of the genes included in the panel are amplified using amplification technology and sequenced on an Illumina instrument to detect single nucleotide variants, insertions, and deletions. This test is designed to detect somatic mutations in the setting of neoplasia, and is not designed to detect germline (heritable) mutations.

Synonyms

11-17, 14, 15, 16), 17), 26, 27), 3, 6), 6), amplicon panel, ASXL1 (CDS), CBL (CDS), CSF3R (Exons 14, DNMT3A (CDS), EZH2 (Exons 15-20), FBXW7 (CDS), FGFR1 (Exons 4, FLT3 (p.D835H), GATA1 (Exons 2-3), GATA2 (Exons 3-5), Hotspot, HRAS (Exon 1-2), IDH1( p.R132), IDH2 (Exon 4), JAK2 (Exon 12, KIT (8-18), KMT2A (CDS), KRAS (CDS), MAP2K1 (Exons 2, MPL (Exon 10), multigene panel, MYD88 (Exon 3-5), next-generation sequencing, NGS, NOTCH1 (Exons 20, NPM1 (Exon 12), NRAS (CDS), partial 18), PDGFRA (Exons 12-18), PHF6 (CDS), PTEN (CDS), RB1 (CDS), RUNX1 (Exon 4-8), SF3B1 (Exon 14-16), SRSF2 (Exon 1), STAG2 (CDS), STAT3 (Exons 20-21), TET2 (CDS), TP53 (CDS), U2AF1 (Exons 2, WT1 (CDS), ZRSR2 (CDS)

Components

Code	Name
OLD	OLD & NOT APPLICABLE

Interpretation

Guidelines

Molecular Testing for Hematopoietic Disorders

Method

Amplification of the stated genetic regions followed by sequencing using an Illumina instrument

Reference Range

See individual components

Ordering & Collection

Specimen Type: Blood, Bone Marrow, Formalin-Fixed Paraffin-embedded Tissue (FFPE)
Collection: Blood: 6 mL blood in LAVENDER top (EDTA) tubeBone Marrow: 1-2 mL in a LAVENDER top (EDTA) tube
Handling Instructions: Samples should be refrigerated and delivered to the Laboratory without delay.

Extracted DNA will be accepted only from CLIA certified laboratories.
Quantity: Requested: Entire tube

Processing

Receiving Instructions: Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood

Samples submitted outside of these ranges will be evaluated for quality using appropriate internal controls and the results qualified appropriately.

Misc Sendout

Performance

Lab Department

Molecular Hemepath- OLD(MHPO)

Frequency

setup weekly, results in 10-12 days.

Available STAT?

Performing Location(s)

Other	Hematopathology, Molecular 206-606-7060 UW Hematopathology Laboratory, G7-800 Fred Hutchinson Cancer Center 825 Eastlake Avenue E. Seattle, WA 98109	Hematopathology, Molecular hours: 7:00 am - 5:30 pm M-F 8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays

Billing & Coding

LOINC: 35474-6
Interfaced Order Code: UOW3087