Heme Single Gene by NGS
General Information
- Lab Name
- Heme Single Gene by NGS
- Lab Code
- HOTSGH
- Epic Ordering
- Heme Single Gene Sequencing
- Description
The Heme Single Gene by NGS (HOTSGH) assay is intended to test a single gene selected from the Heme Amplicon panel. See synonyms field above for the list of offered genes. The regions of the genes included are amplified using amplification technology and sequenced on an Illumina instrument to detect single nucleotide variants, insertions, and deletions. This test is designed to detect somatic mutations in the setting of neoplasia, and is not designed to detect germline (heritable) mutations.
- Synonyms
- 11-17, 14, 15, 16), 17), 26, 27), 3, 6), 6), ASXL1 (CDS), CBL (CDS), CSF3R (Exons 14, DNMT3A (CDS), EZH2 (Exons 15-20), FBXW7 (CDS), FGFR1 (Exons 4, FLT3 (p.D835H), GATA1 (Exons 2-3), GATA2 (Exons 3-5), Hotspot, HRAS (Exon 1-2), IDH1( p.R132), IDH2 (Exon 4), JAK2 (Exon 12, KIT (8-18), KMT2A (CDS), KRAS (CDS), MAP2K1 (Exons 2, MPL (Exon 10), MYD88 (Exon 3-5), next-generation sequencing, NGS, NOTCH1 (Exons 20, NPM1 (Exon 12), NRAS (CDS), partial 18), PDGFRA (Exons 12-18), PHF6 (CDS), PTEN (CDS), RB1 (CDS), RUNX1 (Exon 4-8), SF3B1 (Exon 14-16), single gene, SRSF2 (Exon 1), STAG2 (CDS), STAT3 (Exons 20-21), TET2 (CDS), TP53 (CDS), U2AF1 (Exons 2, WT1 (CDS), ZRSR2 (CDS)
- Components
-
Code Name SGHSD Heme Gene Specimen Description SGHRES Heme Gene Result SGHINT Heme Gene Interpretation SGHM Heme Gene Method
Interpretation
- Method
Amplification of the stated genetic regions followed by sequencing using an Illumina instrument
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Blood, Bone Marrow, Formalin-Fixed Paraffin-embedded Tissue (FFPE)
- Collection
-
Blood: 6 mL blood in LAVENDER top (EDTA) tubeBone Marrow: 1-2 mL in a LAVENDER top (EDTA) tube
- Handling Instructions
Samples should be refrigerated and delivered to the Laboratory without delay.
Extracted DNA will be accepted only from CLIA certified laboratories.
- Quantity
-
requested: Entire tube
Processing
- Processing
Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood
Samples submitted outside of these ranges will be evaluated for quality using appropriate internal controls and the results qualified appropriately.
Performance
- LIS Dept Code
- Molecular Hemepath- OLD (MHPO)
- Performing Location(s)
-
Other Hematopathology, Molecular
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays - Frequency
- setup weekly, results in 10-12 days.
- Available STAT?
- No
Billing & Coding
- CPT codes
- Billing Comments
For additional test/billing information, see Heme Single Gene by NGS CPT codes.
- LOINC
- 35474-6
- Interfaced Order Code
- UOW3081