HCV Genotype by Sequencing

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General Information

Lab Name
HCV Genotype by Sequencing
Lab Code
HCVSEQ
Epic Ordering
HCV GENOTYPE BY SEQUENCING
Description

There are six major genotypes of HCV with numerous characterized subtypes. The most accurate determination of HCV genotype is to sequence the viral genome. This has been done in a variety of ways, including the determination of the entire genome, the 5’ untranslated region, or the core, E1, NS3, or NS5b genes. The most commonly used sequences are from the core and the NS-5 region. The RNA concentration detected with a probability of 95% or greater is 500 IU/mL (2.70 log IU/mL). The sequence is compared to HCV core region sequence to determine the closest related genotype.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For HCV genotyping, see Hepatitis C RNA Genotyping by PCR [HCPCGT].

For HCV quantitation, see Hepatitis C RNA, Quantitative [HCVQNT].

Synonyms
Components

Interpretation

Method

Real-time Polymerase Chain Reaction/Sanger Sequencing

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

Blood: 5 mL blood in PPT (PEARL TOP) tube.

Also Acceptable: 5 mL blood in GOLD SST, RED, or EDTA (LAVENDAR TOP) tube.

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Minimum sample requirement: 1 mL

Handling Instructions
Quantity
requested: See Collection
minimum: 1 mL

Processing

Processing

Blood: Separate serum or plasma from whole blood within 6 hours of collection. Freeze plasma at -10°C or colder and ship on dry ice. Serum or plasma may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Do not freeze/thaw.

Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
Frequency
Once a week.
Available STAT?
No

Billing & Coding

CPT codes
LOINC
32286-7
Interfaced Order Code
UOW2556