Hepatitis B Surface Antigen & Antibody (HBsAg, HBsAb)

A Battery of tests for Hepatitis B virus markers in human sera by the FDA approved Abbott Architect test method:

1. Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb). Results are reported as mIU/mL.
2. Qualitative detection of Hepatitis B virus Surface Antigen (HBSAg).

Reactive HBSAg tests are confirmed by neutralization assay at no charge. Confirmed positives are reflexed to Hepatitis B Quantitative [HBVQNT] for final confirmation with an additional charge.

For HBSAg testing without reflex testing, see Hepatitis B Surface Antigen without PCR reflex for REACTIVE [HBSAGX].

For ordering individual battery components:

HBSAb, see Hepatitis B Surface Antibody (HBsAb) [HBSA].

HBSAg, see Hepatitis B Surface Antigen (HBsAg) with reflex to PCR for REACTIVE [HBSAG].

All Locations: 2 tubes required if reflex testing is ordered

Preferred: 5 mL blood in GOLD SST tube plus 5 mL blood in PEARL PPT tube

Also acceptable: 5 mL blood in ORANGE RST or RED TOP tube plus a 5 mL PEARL PPT tube for reflexive testing.

Note: If no PEARL PPT is received, any reflexive molecular testing can be done on GOLD SST, LAVENDER or RED TOP Tube that has not been on automated lines.

GOLD SST tube: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.

PEARL PPT tube: centrifuge within 6 hours of collection and freeze spun PEARL PPT tube at -20°C. Route frozen PEARL tubes to UW SPS -20°C freezer rack 80. [ok to freeze spun pearl top tubes on gel.]

Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination or are grossly hemolyzed.