Hemoglobin F - HPLC Quantification

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General Information

Lab Name
Hemoglobin F Quant by HPLC
Lab Code
HBF
Epic Ordering
Hemoglobin F Quant by HPLC
Description

Hb F is eluted from the column resin by a programmed buffer gradient of increasing ionic strength.

Hb F level is useful for following hydroxyurea therapy for sickle cell patients.

Hb F measurement is best interpreted in conjunction with other testing in the Thalassemia Reflexive Panel, red cell indices, patient demographics and other clinical factors.

Synonyms
Fetal Hemoglobin, Hb F, Hydroxyurea Follow-up, red cell

Interpretation

Method

High Performance Liquid Chromatography (HPLC)

Reference Range
Units: %
Female Male
AgeRangeAgeRange
0-4m5-90 0-4m5-90
5m5-20 5m5-20
6m-8m<8 6m-8m<8
9m<5 9m<5
10m-3y<3 10m-3y<3
4y-10y<2 4y-10y<2
11y-<1.1 11y-<1.1

Effective date: 09/19/2005

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 mL LAVENDER TOP tube
Pediatric draw: (3) LAVENDER TOP MICROTAINERS

Handling Instructions
Quantity
requested: entire sample
minimum: 1.5 mL whole blood

Processing

Processing

Refrigerate whole blood Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days.

Unacceptable: Other anticoagulants, Frozen specimens.

Performance

LIS Dept Code
Red Cell Disorders (RCD)
Performing Location(s)
HMC Red Cell Disorders
206-520-4600

325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420

Frequency
Monday - Friday
Available STAT?
No

Billing & Coding

CPT codes
83021
LOINC
42246-9
Interfaced Order Code
UOW741
Interfaced Result Code
UOW741