Hemoglobin Electrophoresis (Isoelectric Focusing)
General Information
- Lab Name
- Hb Elect._Isoelectric Focusing
- Lab Code
- HBELEC
- Epic Ordering
- Hemoglobin Electrophoresis (Isoelectric Focusing)
- Description
Hemoglobin electrophoresis alone is not adequate for diagnosing thalassemia. Electrophoresis is limited to identification without fractionation, quantification or interpretation. This test is useful for screening for sickle cell trait for athletes other than those on UW teams. For most red cell disorders, we suggest instead ordering "Hb Disorder Thalassemia Reflex Pnl w/CBC" [HBTHLI]. If a CBC was performed within 30 days, order "Hb Disorder Thalassemia Reflexive Panel" [HBTHAL]. Both panels include hb electrophoresis, reflexive testing for thalassemia and an interpretation.
- Synonyms
- HB ELP, Hemoglobinopathy, hemoglobinopathy, red cell, Sickle Cell Trait, Sickle Cell-Routine, Sickle Dex-Routine
- Components
-
Code Name HER Hb Electrophoresis Result HBDET Hemoglobins Detected HBCOM Hb Elect. Comment
Interpretation
- Method
Isoelectric Focusing Electrophoresis (IEF)
- Reference Range
- See individual components
- Ref. Range Notes
Within Normal Limits
- Guidelines
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL LAVENDER HEMOGARD tube
Pediatric draw: 3 LAVENDER TOP MICROTAINER
- Handling Instructions
For STAT requests from ED location, see [SICPRE] Sickle Screen, Stat, ED only.
- Quantity
-
requested: 3 mL whole blood
minimum: 1.5 mL whole blood
Processing
- Processing
Refrigerate entire sample. If sickle screen is ordered, but not from ED location, login HBELEC.
If request is for Quantitative HbS or Hb Variant Quant., for pre- or post-transfusion, login HBQVAR.
If request is for Quantitative HBF for hydroxyurea therapy, login HBF.
Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days
Unacceptable: Other anticoagulants, Frozen specimen
Performance
- LIS Dept Code
- Red Cell Disorders (RCD)
- Performing Location(s)
-
HMC Red Cell Disorders
206-520-4600325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
- Frequency
- Monday - Friday
- Available STAT?
- No
Billing & Coding
- CPT codes
- 83020
- LOINC
- 43113-0
- Interfaced Order Code
- UOW414