Hemoglobin A2 Quantification by HPLC
General Information
- Lab Name
- Hemoglobin A2 Quant
- Lab Code
- HBA2
- Epic Name
- Hemoglobin A2 Quant
- Description
Hb A2 is eluted from the column resin by a programmed buffer gradient of increasing ionic strength. Hb A2 measurement is best interpreted in conjunction with other testing in the Thalassemia Reflexive Panel and red cell indices and iron supply measurements.
- Synonyms
- HbA2, Hb A2
- Components
Interpretation
- Guidelines
- Method
High Performance Liquid Chromatography (HPLC)
- Reference Range
-
Units: %
Female Male Age Range Age Range 0-6m <3.5 0-6m <3.5 7m- 2.3-3.5 7m- 2.3-3.5 Effective date: 11/02/2012
- Interferences and Limitations
Variant hemoglobins such as Hb S, Hb C, Hb D interfere with measurement of Hb A2, so Hb A2 is not reported in the presence of these variants. Hb E co-elutes with Hb A2 and thus the level reported is the sum of percentages of Hb E and Hb A2.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL LAVENDER HEMOGARD tube
Pediatric draw: 3 LAVENDER TOP MICROTAINER - Quantity
-
Requested: 3 mL whole blood
Minimum: 1.5 mL whole blood
Processing
- Receiving Instructions
Refrigerate whole blood Stability: EDTA: Refrigerated, 7 days. Ambient, 2 days.
Unacceptable: Other anticoagulants, Frozen samples.
- Misc Sendout
Performance
- Lab Department
- Red Cell Disorders(RCD)
- Frequency
- Monday - Friday
- Available STAT?
- No
- Performing Location(s)
-
HMC Red Cell Disorders
206-520-4600325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
Billing & Coding
- CPT Codes
- 83021
- LOINC
- 42245-1
- Interfaced Order Code
- UOW737
- Interfaced Result Code
- UOW737