Adeno/Metapneumo/Rhino Virus PCR Panel

General Information

Lab Name

Lab Code

FUSAMR

Epic Ordering

Adeno/Metapneumo/Rhino Virus PCR Panel

Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® assay for the detection and differentiation of Adenovirus (AdV), human Metapneumovirus (hMPV), and Rhinovirus (RV) using real-time PCR.

The assays targets the AdV Hexon gene, the hMPV nucleocapsid gene and RV 5’ UTR . Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.

Synonyms

Components

Code	Name
FUSSD	Adeno/Metapneumo/Rhino Virus PCR Spec
FUSADV	Adenovirus Result
FUSACT	Adenovirus Ct Value
FUSMPV	Metapneumovirus Result
FUSNCT	Metapneumovirus Ct Value
FUSRHV	Rhinovirus Result
FUSRCT	Rhinovirus Ct Value
FAMRIN	Adeno/Metapneumo/Rhino Virus PCR Interp

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range

See individual components

Guidelines

Guidelines for selecting respiratory virus testing

Ordering & Collection

Specimen Type: Nasopharyngeal swab, Oropharyngeal swab, Anterior Nares swab, Broncho alveolar lavage (BAL) and tracheal aspirate.
Collection: Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.

Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.

Minimum sample requirement: 0.75 mL
Handling Instructions: Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.
Quantity: requested: 1mL
minimum: 750 µl

Processing

Processing: Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Central Lab Virology (RVIR)

Performing Location(s)

CL	Virology, Renton 206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356	206-685-6656 opt 4

Frequency

Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.

Available STAT?

Billing & Coding

CPT codes: 87631
LOINC: 81655-3
Interfaced Order Code: UOW4284