FLT3 TKD D835/I836 Mutations
General Information
- Lab Name
- FLT3 TKD D835/I836 Mutations
- Lab Code
- FLTTKD
- Epic Ordering
- FLT3-TKD D835/I836 Mutations
- Description
This assay detects mutations in the FLT3 gene at codons D835/I836 of the tyrosine kinase domain (FLT3 TKD). Evaluation for FLT3 point mutations in the TKD is indicated at diagnosis of acute myeloid leukemia and may provide prognostic information and direct treatment. Positive FLT3 TKD results may be further evaluated by next-generation sequencing to identify the specific mutation upon request. This is a qualitative and assay does not detect other point mutations throughout the TKD nor the FLT3 internal tandem duplication (FLT3-ITD).
Limit of Detection: 2.5%
Hematopathology test request form found at Request Form
- Forms & Requisitions
- Synonyms
- FLT3 D835 and I836, FLT3 D835/I836, FLT3 point mutation, FLT3 TKD, FLT3 tyrosine kinase domain, Heme Amplicon Panel by NGS (HOTSPT), Heme Single Gene by NGS (HOTSGH), UW Oncoplex Cancer Gene Panel (OPX)
- Components
-
Code Name TKDSD FLT3 TKD Specimen Description TKDRES FLT3 TKD Result TKDINT FLT3 TKD Interpretation TKDMTH FLT3 TKD Method TKDGRN FLT3 TKD Case Number
Interpretation
- Method
PCR amplification of DNA followed by restriction enzyme and capillary electrophoresis for size fractionation
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Blood, Bone Marrow, Tissue - Fresh, frozen or FFPE/PET (Blocks), Fluids, Slides, Sorted Cells
- Collection
-
Preferred:
Blood: 6mL in Lavender Top (EDTA)Bone Marrow: 1-2mL in Lavender Top (EDTA)
Tissue, Fluids or Sorted cells: Sterile container in RPMI
Formalin-fixed paraffin embedded tissue (FFPE/PET) block
Slides: 10 unstained
Accepted:
Green Tops (Heparin), while green tops are accepted for testing, there is documentation that heparin can interfere with some PCR assays.Extracted DNA from CLIA certified laboratory.
Formalin-fixed paraffin embedded tissue (FFPE/PET)
Tissue from curls cut from FFPE/PET blocks or stained cover-slipped slides
Unacceptable:
Specimens received beyond stability limit.
Specimens with low volume or inadequate WBC
Decalcified or unacceptable fixatives - Forms & Requisitions
- Handling Instructions
- Deliver samples to Hematopathology as soon as possible.
- If sample is older than 48 hours, please store at 2-6°C upon receipt and send refrigerated.
- Samples stored at room temperature or refrigerated older than 7 days will be rejected.
- Frozen samples should be shipped on dry ice.
- Quantity
-
requested: Blood: 6mL EDTA/BMA: 1-2mL EDTA/Tissue Blocks: Adequate size to cut 10-20 curls/Slides: 10 unstained
minimum: Blood: 1mL EDTA/ BMA: 0.5mL EDTA/Tissue Blocks: 5 curls/Slides: 5 slides
Processing
- Processing
Send specimens to Hematopathology – Molecular department for processing.
The stability limit for Blood and Bone Marrow is 7 days, samples beyond this limit will be rejected.
Performance
- LIS Dept Code
- Molecular Hematopathology (MHP)
- Performing Location(s)
-
Other Hematopathology
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology hours:
5:30 am - 6:30 pm Monday through Saturday
7:30 am - 4:00 pm Sunday, and with reduced staffing on major holidaysOther Hematopathology, Molecular
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays - Frequency
- Monday, Wednesday, Friday: results available within 24hrs. EVALHD reflexed or add-on orders may take up to 5 days to complete.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 81246
- LOINC
- 72520-0
- Interfaced Order Code
- UOW3325