Protein Electrophoresis Reflexive Panel
General Information
- Lab Name
- Protein Electrophoresis Panel
- Lab Code
- ELPP
- Epic Name
- Protein Electrophoresis Reflexive Panel
- Description
Purpose: This test can be used to screen for and identify a monoclonal protein (M-spike).
The initial test performed is serum protein electrophoresis. Samples with a monoclonal band present on protein electrophoresis that has previously been identified in this laboratory will be resulted with the concentration of the known M-spike. Samples demonstrating previously unidentified protein bands of concern will be reflexively tested by immunofixation, which generates an additional result and charge. If a monoclonal band is identified by immunofixation, its concentration will be reported.
Serum free light chain testing (FLC) testing is available as a separate order, and is not included in this panel.- Synonyms
- Gammopathy, Immunofixation, M-Protein, MGUS, Monoclonal, Monoclonal antibody, Multiple myeloma, Myeloma, Serum electrophoresis, Serum protein electrophoresis, SPEP, Waldenstrom
- Components
-
Code Name ETP Total Protein (Beckman) ETPRD Total Protein (Roche) EALB Albumin EA1 Alpha 1 EA2 Alpha 2 EB Beta EG Gamma INTRPE Electrophoresis Interp: MC1ID Monoclonal 1 ID, Serum MC1QN Monoclonal 1 Quant, Serum MC2ID Monoclonal 2 ID, Serum MC2QN Monoclonal 2 Quant, Serum
Interpretation
- Guidelines
- Method
Agarose Electrophoresis (AEP)
- Reference Range
- See individual components
- Interferences and Limitations
Hemolysis may falsely elevate alpha-2 and beta components.
Stability: 1 week refrigerated; stable long-term if frozen.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL blood in RED TOP or GOLD SST tube.
Pediatric: 2 full GOLD or RED TOP MICROTAINERS. - Quantity
- Requested: 0.5 mL serum ELP; 0.5 mL for TP
Processing
- Receiving Instructions
Refrigerate serum.
Make two aliquots: 0.5 mL to IMM for ELP and 0.5 mL to CHEM for ETP or ETPRD.OK to share ELP and IFIX on one tube if short sample. Forward shared pink slip to Immunology.
Outside Laboratory: If Total Protein is provided, please free text "TP provided" after test ordered and provide a copy of the slip to Panel Bench and Immunology.
- Misc Sendout
Performance
- Lab Department
- Immunology(IMM)
- Frequency
- Monday-Friday
- Available STAT?
- No
- Performing Location(s)
-
CL Immunology Lab
206-520-4600Room# 573
1601 Lind Ave SW
Renton, WA 98057Hours of Operation: Monday–Friday, 8:00 AM–5:00 PM PST
Phone: (206) 485-9268CL Renton Core Lab
206-520-4600Clinical Lab, Room 170
1601 Lind Ave SW
Renton, WA 98057HMC Chemistry, Automated
206-520-4600325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT Chemistry, Automated
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195UW-NW Main Lab
206-668-1344UW Medical Center – Northwest
1550 N 115th Street, A200
Seattle, WA 98133
Billing & Coding
- CPT Codes
- 84165x2
- LOINC
- 24351-9
- Interfaced Order Code
- UOW2008