BRCA1&2 Analysis

General Information

Lab Name

BRCA1&2 Analysis

Lab Code

BRCA12

Epic Name

BRCA 1&2 Analysis

Description

BRCA1 & BRCA2 testing is useful for the evaluation of patients who are suspected to have hereditary breast and ovarian cancer. Testing is performed using next-generation sequencing to detect most mutations in BRCA1 and BRCA2. The assay completely sequences all exons of these genes AND detects large deletions, duplications and mosaicism.

For patients with a suspected hereditary cancer syndrome, including hereditary breast and ovarian cancer syndrome, see BROCA Cancer Risk Panel [BROCA].

Forms & Requisitions

Requisition Form and Ordering Instructions:

Fill out a Genetics Requisition
Check "BRCA 1/2 - Complete Analysis"

Preauthorization Form (preauthorization is only done for providers who are external to the UW system)

Synonyms

BART, BRCA1&2 deletion and duplication testing, BRCA1&2 Sequencing

Components

Code	Name
BRCRE	BRCA1&2 Result
BRCIN	BRCA1&2 Interpretation
BRCCH	BRCA1&2 Clinical History
BRCMT	BRCA1&2 Method
BRCDI	BRCA1&2 Director

Interpretation

Guidelines

Next-Generation Sequencing Panels Preauthorization

Method

Next-generation sequencing

Reference Range

See individual components

Ordering & Collection

Specimen Type

Peripheral Blood, cultured cells from skin biopsy, purified DNA from peripheral blood or cultured cells, and saliva

Collection

BLOOD:

10 mL whole blood in LAVENDER TOP EDTA tube.
Also acceptable: YELLOW TOP ACD tube, purified DNA from peripheral blood or cultured cells.

SKIN BIOPSY:

Collection and transport: Obtain 2-4 mm punch biopsy of skin sample under sterile conditions and place in transport media (e.g. Alpha-MEM media, RPMI). Transport media can be supplied by the lab; call 206-598-4488 to request. If transport media is not available, the following media are acceptable alternatives if shipping time will not exceed 24 hours: lactated Ringer's solution, viral transport medium, or sterile saline. DO NOT USE formaldehyde, formalin, alcohol, or 5% dextrose, or tissue culture medium buffered with bicarbonate.

CULTURED CELLS:

(2) T23 or (1) T75 flask (minimum 1-T25 flask)*.

*Prenatal testing requires concomitant testing for maternal cell contamination (see Online Test Guide Maternal Cell Contamination, Fetal [MCC] for ordering and specimen requirements)

Forms & Requisitions

Requisition Form and Ordering Instructions:

Fill out a Genetics Requisition
Check "BRCA 1/2 - Complete Analysis"

Preauthorization Form (preauthorization is only done for providers who are external to the UW system)

Handling Instructions

Ship specimen at room temperature for overnight delivery.

Blood specimens can be held for up to 7 days before shipping if refrigerated.

Ship specimens to:

UW MEDICAL CENTER

LABORATORY MEDICINE - GENETICS LAB

1959 NE PACIFIC ST, ROOM NW220

SEATTLE, WA 98195-7110

Quantity

Requested: Entire sample
Minimum: 5 ml whole blood

Processing

Receiving Instructions: Blood: Refrigerate whole blood

Unacceptable Conditions: Frozen or clotted specimens

Stability (collection to initiation of testing): Ambient: 5 days; Refrigerated: 7 days; Frozen: Unacceptable

Purified DNA: Refrigerate DNA specimens. Frozen is acceptable.

SALIVA:
Contact laboratory for validated collection kit.

Misc Sendout

Performance

Lab Department

Genetics(GEN)

Frequency

Typical Turnaround: 3 weeks *Turn around times may vary based on factors such as tissue acquisition and insurance preauthorization.

Available STAT?

Performing Location(s)

UW-MT	Genetics Attention: Genetics Lab Clinical lab, Room NW220 University of Washington Medical Center 1959 NE Pacific Street Seattle, WA 98195 Tel: 206-598–6429 M–F (7:30 AM–4:00 PM) Fax: 206-616-4584 Lab email: cgateam@uw.edu Tel (EXOME only): 206-543-0459	Faculty Jillian Buchan, PhD, FACMG Runjun Kumar, MD, PhD Regina Kwon, MD, MPH Christina Lockwood, PhD, DABCC, DABMGG Abbye McEwen, MD, PhD Colin Pritchard, MD, PhD Vera Paulson, MD, PhD Eric Konnick, MD, MS He Fang, PhD

Billing & Coding

LOINC: 59041-4
Interfaced Order Code: UOW2858
Billing Comments: For billing information, see BRCA1&2 Analysis Billing

For pricing information, contact Client Support Services 206-520-4600 or 800-713-5198.

Billing and Insurance Pre-Authorization

We offer insurance pre-authorization services (preauthorization is only done for providers who are external to the UW system).

Email: gpab@uw.edu or call 1-855-320-4869 for more information.

Genetics Preauthorization Form