Anti Endomysial, IgA (by IFA)

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General Information

Lab Name
Anti Endomysial, IgA
Lab Code
AEMYA
Epic Ordering
Anti Endomysial, IgA
Description

The presence of IgA endomysial autoantibodies by indirect immunofluorescence is suggestive of active gluten sensitive enteropathies such as celiac disease. Results are primarily useful for diagnosis; they may also be used for monitoring response to treatment with a gluten-free diet, but results are semi-quantitative.

References

Bürgin-Wolff, et al., Antigliadin and antiendomysium antibody determination for coeliac disease. Arch Dis Child. (1991) 66:941.

Synonyms
Celiac, Celiac Ab, Endomysium, Sprue

Interpretation

Method

Indirect immunofluorescence (IFA) using INOVA monkey esophagus tissue to detect IgA autoantibodies.

Reference Range
Female Male
AgeRangeAgeRange
0-Negative [NRN] 0-Negative [NRN]

Effective date: 04/01/2010

Ref. Range Notes

Established by testing healthy blood donors during method validation.

Interferences and Limitations

Anti-smooth muscle antibodies may obscure endomysial staining. The test cannot be used for patients with IgA deficiency.

Grossly hemolyzed or lipemic serum may produce increased nonspecific background staining of substrate.

References

Bürgin-Wolff, et al., Antigliadin and antiendomysium antibody determination for coeliac disease. Arch Dis Child. (1991) 66:941.

Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in RED TOP or SST tube

Handling Instructions

Instructions for Outside Clients: Freeze serum at -20°C

Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C: Long Term

Quantity
requested: 1 mL serum
minimum: 0.3 mL serum

Processing

Processing

Freeze serum at -20°C

Note: If Anti Endomysial is requested without specifying method (i.e. IFA or EIA), then order: AEMYA

Note2: AEMYA may be reflexively added on to CELRP orders if indicated.

Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C: Long Term

Performance

LIS Dept Code
Immunology (IMM)
Performing Location(s)
UW-MT Immunology
206-520-4600

Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Frequency
Wednesday, Friday. Negative results same day. Positive results on next run.
Available STAT?
No

Billing & Coding

CPT codes
86231
LOINC
46126-9
Interfaced Order Code
UOW866
Interfaced Result Code
UOW866