Hepatitis C Viral RNA Genotype 1 NS3 Drug Resistance

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General Information

Lab Name
Lab Code
633
Description

Clinical Significance: This assay may be used to detect grazoprevir, simeprevir and paritaprevir resistance-associated NS3 mutations in genotype 1 NS3 protease inhibitor treatment-experienced patients and also the Q80K polymorphism in patients being considered for a simeprevir-containing regimen.

IDSA HCV Resistance Guidelines

Note: HCV Genotype 1 is required for this test.

Synonyms
Boceprevir Resistance, NS3 Protease Mutations, NS3 Subtype, Q80K Polymorphism, Simeprevir Resistance, Telaprevir Resistance

Interpretation

Method

Polymerase Chain Reaction (PCR), Sequencing

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in LAVENDER TOP (EDTA) tube (preferred)

Also Accepted: 6 mL blood in a PEARL TOP (PPT Potassium EDTA), RED TOP tube, or GOLD TOP (SST)

Handling Instructions
Quantity
requested: 2.0 mL plasma/serum
minimum: 0.6 mL plasma/serum

Processing

Processing

Centrifuge sample and transfer plasma/serum to a separate, SCREW-CAPPED plastic vial within 24 hours of collection. Freeze at -20°C.

Login: SEND1-;FREEZE

  • RSNDT1: QSTID
  • RSTYP1: PLS (or SERUM)
  • RTSRQ1: ;HCV RNA Genotype 1 NS3 Drug Resistance (Quest Test 90924)

Sendouts, order Quest Test: 90924

Stability: Frozen (preferred) 42 days; Refrigerated: 72 hours; Ambient: Unacceptable.

Reject due to:

  • Gross hemolysis
  • Gross lipemia
  • Specimens collected in heparin anticoagulant

Performance

LIS Dept Code
Performing Location(s)
Sendout Quest Diagnostics Infectious Diseases, Inc.
800-445-4032

33608 Ortega Highway
San Juan Capistrano, CA 92675
Frequency
Set up: Monday. Report Available: 4-11 days.
Available STAT?
No

Billing & Coding

CPT codes
Billing Comments

CPT: 87902

LOINC