Chagas TESA Immunoblot (Sendout)

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General Information

Lab Name
Lab Code
1169
Epic Name

Order using Lab Undefined Order [LABUND]
External Test Id
CHT21
Description

Test Description: Chagas TESA immunoblot is an Kephera-developed assay utilizing Trypomastigote Excreted/Secreted Antigens. Native TESA is harvested and enriched from parasite culture medium. TESA is subjected to gel electrophoresis to separate proteins by size, followed by transfer to a nitrocellulose membrane. When a serum sample is added to the membrane strip, specific anti-T. cruzi antibodies will bind to protein antigens immobilized on the membrane at specific locations. After washing to remove unbound antibodies, an anti-human IgG enzyme conjugate is added followed by a substrate that will change color if the conjugate has bound to antigen-antibody complexes. The presence or absence of a band at a specific location on the strip is interpreted visually.

Ordering Guidance: This test is the recommend tie-breaker to resolve discordant results from the Trypanosoma cruzi Antibody Panel (Sendout) [RCRUZI].

Ordering Requirements: Laboratory Medicine Resident (LMR) approval is required.

References
Forms & Requisitions
Synonyms
CHT21, Kephera, T. cruzi, Trypanosoma cruzi
Components

Interpretation

Guidelines
Method

Immunoblot

Reference Range
References

Ordering & Collection

Specimen Type
Blood
Collection

3-5 mL in RED TOP tube or GOLD SST

Also Accepted: LAVENDER (EDTA)

Forms & Requisitions
Approval Required
Laboratory Medicine Resident (LMR) approval is required to order this test.
Handling Instructions

Outside Laboratories: Centrifuge sample and aliquot serum or plasma into a separate plastic vial. Freeze serum/plasma at -20°C to extend stability while awaiting shipment. Transport on dry ice.

Stability: Refrigerated (preferred): 96 hours; Frozen: Accepted for samples >96 hours (avoid freeze-thaw cycles); Ambient: 24 hours.

Reject Due To: Hemolyzed, lipemic, icteric, or cloudy samples.

Quantity
Requested: 1 mL serum or plasma
Minimum: 0.5 mL serum or plasma

Processing

Receiving Instructions

Centrifuge sample and aliquot serum or plasma into a separate plastic vial. Refrigerate serum/plasma.

Login: SPMSND-;REFRIG SERUM

  • MSNDTO: KEPHRA
  • MSPTYP: <Enter appropriate ETC for specimen type received>
    • Serum: SRM
    • Plasma: PLS
  • MTSTRQ: ;Chagas TESA Immunoblot (Kephera Test CHT21)

Sendouts:

  • Order Kephera Test: CHT21
  • Complete a Kephera Diagnostics requisition (use the account-specific requisition located in OneDrive).
  • Ensure that the sample label includes the patient's date of birth.
  • Send samples Monday through Wednesday via FedEx Priority Overnight.
    • Samples can be shipped on Thursday for Friday delivery, but it is not preferred.
    • Saturday delivery is not available.
  • If samples are not expected to arrive at Kephera within 96 hours of collection, freeze serum/plasma at -20°C while awaiting shipment and send on dry ice.

Stability: Refrigerated (preferred): 96 hours; Frozen: Accepted for samples >96 hours (avoid freeze-thaw cycles); Ambient: 24 hours.

Reject Due To: Hemolyzed, lipemic, icteric, or cloudy samples.

Misc Sendout

Performance

Lab Department
Frequency
Turnaround Time: ≤14 days from sample receipt at Kephera Diagnostics.
Available STAT?
No
Performing Location(s)
Sendout Kephera Diagnostics
774-777-3558

1 Grant Street, Suite 300
Framingham, MA 01702

Fax: 774-777-3559
Email: info@kephera.com

Billing & Coding