Acylcarnitine Profile, Blood Spots (Sendout)

single page view

General Information

Lab Name
Lab Code
1146
Epic Name

Order using Lab Undefined Order [LABUND]
External Test Id
LAB2702
Description

Clinical Utility: Analysis of acylcarnitines is useful in diagnosis of defects in fatty acid beta-oxidation and various disorders of organic acid metabolism. Acylcarnitine analysis is often used in conjunction with urine organic acid analysis in the diagnosis of these disorders and in followup of presumptive positive newborn screening results.

    Test Description: Measurement of acylcarnitines by ESI-MS/MS using stable isotope internal standards. Free carnitine is also reported. To order Free/Total Carnitine only, see separate test Carnitine Group (Sendout) [RCARNG].

      Ordering Requirements: Include relevant clinical information (drug therapy, family history, etc.) in the Epic order comment to ensure proper interpretation.

        Ordering Note: Plasma is the preferred sample type, especially for follow-up testing of an abnormal newborn screen. See Acylcarnitine Profile, Plasma/Serum (Sendout) [RACYLP]. Dried blood spot testing should only be performed on neonates when blood volume is a concern.

        Forms & Requisitions
        Synonyms
        Acylcarn, LAB2702
        Components

        Interpretation

        Method

        Tandem mass spectrometry

        Reference Range
        Ref. Range Notes

        Blood Spot Reference Ranges

        Ordering & Collection

        Specimen Type
        Blood
        Collection

        Materials: Newborn Screening Collection Cards are available through Materials Management. Specify Stock Item: #61118. The laboratory does not stock collection cards.

          Collection:

          • Cleanse the site with a sterile alcohol pad and allow alcohol to dry completely.
          • Perform capillary puncture using a sterile lancet. Wipe the first drop of blood with a sterile guaze pad.
          • Fold back the protective flap on the card to expose filter paper. Do not touch or handle the filter paper before or after applying blood.
          • Lightly touch filter paper to the large blood drop, allowing blood to soak through the filter paper and saturate the circle completely with a single application. Apply blood to one side of the paper only.
          • Very gentle, intermittent pressure may be applied to the area surrounding the puncture site. Do not squeeze the puncture site.
          • Continue with collection until at least 3 of the 5 circles are completely filled with blood.
          • Allow collection card to dry completely on a clean, dry, non-absorbent surface, then replace the protective flap and store in the provided envelope.
            • Do not store samples in plastic.
          Forms & Requisitions
          Quantity
          Requested: 3 full circles of blood on a newborn screening card
          Minimum: 3 full circles of blood on a newborn screening card

          Processing

          Receiving Instructions

          Store and transport blood collection cards at ambient temperature.

          Login: SEND1-;AMBIENT

          • RSNDT1: SEACHL
          • RSTYP1: BLSP
          • RTSRQ1: ;Acylcarnitine Profile, Blood Spots (SEACHL Test LAB2702)

          Sendouts:

          • Order SEACHL Test: LAB2702
          • If clinical information (drug therapy, family history) is included with the order (optional), print the Epic requisition and send it with a completed SEACHL Biochemical Genetics requisition.
          • Send cards at ambient temperature via DLMP or Delivery Express courier.

          Stability: Ambient (required): 5 days.

          Misc Sendout

          Performance

          Lab Department
          Frequency
          Performed: Monday, Thursday. Reported: 3-7 days from sample receipt at Seattle Children's Hospital Laboratory.
          Available STAT?
          No
          Performing Location(s)
          Sendout Seattle Children's Hospital Laboratories
          206-987-2617

          4800 Sand Point Way NE
          Seattle, WA 98105

          Room Number: FB.2.441

          Billing & Coding

          Billing Comments

          CPT: 82017