Prothrombin (F2) G20210A Variant (Sendout) [Expedited Living Donor Workup]

General Information

Lab Name

Prothrombin (F2) G20210A Variant (Sendout)

Lab Code

RPROTM

Epic Ordering

Prothrombin (F2) G20210A Variant (Sendout)

External Test Id

0056060

Description

Test Information: Detects prothrombin NM_000506.4 c.20210G>A *97 G>A (legacy nomenclature: G20210A) pathogenic variant.

Ordering Restrictions: This test is approved for UWMT Liver Transplant Clinic only and should be used exclusively for screening liver donor candidates. For orders from other locations, refer to in-house test Prothrombin DNA Screen [PRODS].

References

ARUP Technical Information: Prothrombin (F2) c.*97G>A (G20210A) Pathogenic Variant

Synonyms

0056060, F2 DNA assay, Factor II (PT) 20210 Variant, Prothrombin (F2) c.*97G>A (G20210A) Pathogenic Variant, Prothrombin Gene Analysis

Components

Code	Name
RPROTS	Prothrombin (F2) G20210A Specimen
RPROTV	Prothrombin (F2) G20210A Variant Result

Interpretation

Method

Polymerase Chain Reaction/Fluorescence Monitoring

Reference Range

See individual components

Ref. Range Notes

Result:	Variant(s) Detected:	Interpretation:
Negative	No copies of the variant detected	Does not exclude other hereditary risk factors.
Heterozygous	One copy of the variant detected	Confers a two- to fourfold increase in thrombotic risk in the absence of other risk factors¹
Homozygous	Two copies of the variant detected	Confers an increased thrombotic risk (not well characterized at this time)

Interferences and Limitations

Limitations:

Diagnostic errors can occur due to rare sequence variations.
F2 gene variants other than NM_000506.4 c.20210G>A, *97 G>A will not be detected.

References

ARUP Technical Information: Prothrombin (F2) c.*97G>A (G20210A) Pathogenic Variant

Ordering & Collection

Specimen Type: Blood
Collection: 3 mL in LAVENDER TOP (EDTA) tube

Also Accepted: Yellow (ACD Solution A or B), Pink (K2EDTA)
Approval Required: Approved for UWMC Liver Tranplant Clinic only as part of an expedited living donor workup.
Handling Instructions: Outside Laboratories: This test is available to UW Medicine providers only. Refer to Prothrombin DNA Screen [PRODS] for testing available within DLMP.
Quantity: requested: 3 mL whole blood
minimum: 1 mL whole blood

Processing

Processing

Refrigerate whole blood.

Sendouts:

Order ARUP Test: 0056060.
Interfaced: Yes. [Interface: 602; Department: ARPR]

Stability: Refrigerated (preferred): 1 week; Ambient: 72 hours, Frozen: 1 month.

Unacceptable Conditions: Plasma or serum; collection of specimen in sodium heparin tubes.

Performance

LIS Dept Code

Sendouts ARUP Lab (RF) (ARPR)

Performing Location(s)

Sendout	ARUP Laboratories, Inc. 800-522-2787 500 Chipeta Way Salt Lake City, UT 84108

Frequency

Performed: Sunday - Saturday. Reported: 2-5 days.

Available STAT?

Billing & Coding

CPT codes: 81240
LOINC: 24475-6