Lupus Anticoagulant Group and Anti phospholipid Panel with Interpretation

Direct thrombin inhibitors such as hirudin (Refludan), bivalirudin (Angiomax), dabigatran (Pradaxa), argatroban (Novastan) or anti-Xa drugs such as rivaroxaban (Xarelto), apixaban (Eliquis) and edoxaban (Savaysa), frequently interfere with the Lupus anticoagulant assay and lead to falsely positive results.

For patients on direct factor Xa inhibitors (Apixaban, Rivaroxaban, Edoxaban), order Hexagonal Phospholipid assay (HEXPI) instead.

UW-MT: Take Blue tops to UW-MT Coag for processing. Coag tech will freeze TWO plasma aliquots (minimum 1 mL each). One aliquot will go to Immunology and one aliquot should be sent to HMC Coag. If one Blue top is received, make TWO aliquots (minimum 1 mL each). If only 1.0 mL plasma aliquot is available, send aliquot to HMC Coag first with pink shared slip.

HMC: Take both Blue tops to HMC Coag Lab for processing. Coag tech will freeze TWO plasma aliquots (minimum 1 mL each). One aliquot will go to Immunology and one aliquot to HMC Special Coag. If one Blue top is received, make TWO aliquots (minimum 1 mL each). If only 1.0 mL plasma aliquot is available, send aliquot to HMC Special Coag first with pink shared slip.

Outside Laboratory: Centrifuge Blue tops for 10 minutes, remove plasma & respin plasma for another 10 minutes. Decant and freeze TWO plasma aliquots (minimum 1.0 mL each) at -20°C to -80°C. Ship frozen on dry ice.

SPS Note: If two plasma tubes are received, send one to Immunology for APLSP and one to HMC Coag for LUPI. If one serum and one plasma are received, change order to APLSP and LUPI. Use serum tube for APLSP and plasma tube for LUPI. If only one plasma tube is received, order LUPGI2 and send to HMC Coag first with pink shared slip.